高效液相色谱法测定花地灌肠液中芦丁的含量  

Determination of Rutin in Hua Di Enema by HPLC

作　　者：李咏梅[1] 周小忠 钟淑娟[1] 罗思婧 江伟鹏 

机构地区：[1]广州医科大学附属第五医院,广东广州510700 [2]广州花海药业股份有限公司,广东广州510370 [3]广州固志药物研究有限公司,广东广州510370

出　　处：《今日药学》2015年第6期407-408,416,共3页Pharmacy Today

基　　金：建设中医药强省立项资助科研课题(20141181)

摘　　要：目的建立高效液相色谱法（HPLC）测定花地灌肠液中芦丁的含量。方法色谱柱为Thermo ODS-2（4.6 mm×250 mm,5μm）,流动相为甲醇-1%甲酸（32∶68）,检测波长为257 nm,流速为1.0 m L/min。结果芦丁线性范围为0.827 4-4.137μg（r=0.999 9）,平均回收率为99.7%（RSD=1.0%）。结论该方法简便、快速、分离度好、结果稳定,可用于花地灌肠液的质量控制。OBJECTIVE To establish an HPLC method for the determination of rutin in Huadi enema. METHODS The separation was performed on Thermo ODS-2（4. 6 mm × 250 mm,5 μm） column with Methanol- 1% formic acid（32∶ 68） as a mobile phase,the detection wavelength was at 257 nm,and the flow rate was 1. 0 m L / min. RESULTS The linear range of rutin was within0. 827 4- 4. 137 μg（ r = 0. 999 9）. The average recovery of rutin was 99. 7%（ RSD = 1. 0%）. CONCLUSION The method is simple,rapid and capable of producing good separation results. It can be applied to the quality control of Huadi enema.

关 键 词：高效液相色谱法 花地灌肠液 芦丁 含量 

分 类 号：R284[医药卫生—中药学]