PSF蛋白及其在眼科的应用研究  

Application of PSF in ophthalmology

作　　者：漆晨[1,2,3] 郭如如 东莉洁[1,2,3] 张晓敏[1,2,3] 李筱荣[1,2,3] 

机构地区：[1]天津医科大学眼科医院,300384 [2]天津医科大学眼科研究所,300384 [3]天津医科大学眼视光学院,300384

出　　处：《国际眼科纵览》2015年第3期195-199,共5页International Review of Ophthalmology

基　　金：国家自然科学基金（31100991）

摘　　要：多聚嘧啶束结合蛋白相关剪接因子（polypyramidine tract binding protein-associated splicing factor,PSF）是存在于细胞核内且参与多种细胞反应的多功能蛋白质.经玻璃体腔注射重组腺相关病毒包装的PSF蛋白（rAAV-PSF）可有效抑制氧诱导视网膜病变模型小鼠视网膜新生血管的形成,下调血管内皮生长因子（vascular endothelial growth factor,VEGF）的表达,从而控制新生血管的增生;PSF蛋白通过募集Hakai复合物,下调VEGF启动子的转录激活,从而抑制胰岛素样生长因子（in-sulin-like growth factor,IGF-1）刺激的视网膜血管内皮细胞增生.此外,PSF蛋白通过调控细胞因子信号抑制物3的表达,间接影响JAK/STAT信号通路的活化状态,而在斑马鱼视神经再生中发挥重要作用.Polypyrimidine tract-binding protein-associated splicing factor （PSF） is a multifunctional protein involved in multiple distinct cellular responses.The latest evidence showed that intravitreal administration of recombinant adeno-associated virus-loaded PSF （rAAV-PSF） normalizes the retinal vessel and inhibits vascular endothelial growth facotr （VEGF） expression in an oxygen-induced retinopathy （OIR） mouse model.Therefore,it ameliorates retinal neovascularization （RNV） in OIR mouse model.On the other hand,it has been shown that PSF can repress the proliferation of human retinal capillary endothelial cells （HRCECs） and the transcriptional activation of VEGF stimulated by insulin-like growth factor-1 （IGF-1） via recruitment of the Hakai complex and delineates a novel regulatory mechanism of IGF-1/VEGF signaling.In addition,PSF regulates the expression of suppressor of cytokine signaling （SOCS3） and attenuate JAK/STAT phosphorylation status to control optic nerve regeneration in zebrafish.

关 键 词：PSF蛋白 视网膜新生血管 视神经再生 转录调控 

分 类 号：R817.4[医药卫生—影像医学与核医学]