自贡地区金葡菌qacA基因检测  

Epidemiology of disinfectant-resistant gene qacA in Staphylococcus aureus in Zigong Area

作　　者：孙卫东[1] 章成[1] 周廷权 杨军[1] 

机构地区：[1]四川省自贡市第四人民医院老年科,四川自贡643000 [2]重庆医科大学附属第一医院感染科,重庆630014

出　　处：《医药前沿》2015年第17期26-28,共3页Journal of Frontiers of Medicine

摘　　要：目的：了解自贡地区近两年临床分离的金黄色葡萄球菌中耐药基因qacA的携带情况。方法：采用CLSI推荐的30mg头孢西丁纸片法进行耐甲西林金黄色葡萄球菌（MRSA）鉴定，采用PCR技术检测qach基因。结果临床分离的143株金黄色葡萄球菌中65株为MRSh（其中qach基因阳性率为10．8％），共有9株qach基因呈阳性（阳性率为6．3％）。结论：自贡地区临床分离的金黄色葡萄球菌中qacA基因携带率低于国外文献报道，与国内文献报道一致。Objective To investigate the clisinfectant--resistant-gene qacA in Staphylococcus aureus isolates from Zigong Municipal of Sichuan. Methods A total of 143 S,aureus strains were isolated. Disk diffusion method was used to identify methiciUin-resistant S. aureus（MRSA）. PCRwas employed to analyze the prevalence of qacA gene in these strains. Results Of the 143 S. aureus strains,9 were positive for qacA gene. The prevalence of qacA in these strains was 6.3%. The prevalence of qacA gene in MRSA was10.8%. Conclusion The prevalence of qacA gene in the clinical isolates of S.aureus in the hospitals of Zigong Municipal is lower than that of the reports at abroad and it＇ sconsistent with domestic reportes.

关 键 词：金黄色葡萄球菌 耐甲氧西林金黄色葡萄球菌 耐药基因qacA 

分 类 号：R446[医药卫生—诊断学]