有机养殖模式下中华绒螯蟹抗氧化防护和溶菌酶的活性  

Antioxidant Defenses and Lysozyme Activity in Chinese Mitten-handed Crab Eriocheir sinensis Farmed in Organic Farming Pattern

作　　者：吴凡[1] 赵燕静[1] 管卫兵[2] 蒋昕 周传江[1] 孔祥会[1] 

机构地区：[1]河南师范大学水产学院,河南新乡453007 [2]上海海洋大学海洋科学学院,上海201306

出　　处：《水产科学》2015年第7期418-422,共5页Fisheries Science

基　　金：河南省国际合作与人才项目(144300510017);河南省水产学重点学科项目(2012)

摘　　要：比较了有机和常规养殖模式下,体质量165~175g中华绒螯蟹鳃、肝胰脏和肌肉中溶菌酶活性、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性以及丙二醛含量。结果显示,有机养殖模式下,中华绒螯蟹肝胰脏和肌肉中超氧化物歧化酶和过氧化氢酶活性显著高于常规养殖模式（P〈0.05）,但鳃中超氧化物歧化酶和过氧化氢酶活性与常规养殖组差异不显著（P〉0.05）;鳃、肝胰脏和肌肉中谷胱甘肽过氧化物酶活性均显著高于常规养殖模式（P〈0.05）;鳃、肝胰脏和肌肉中丙二醛含量显著低于常规养殖模式（P〈0.05）。有机养殖模式下中华绒螯蟹肝胰脏中的溶菌酶活性显著高于常规养殖组（P〈0.05）。试验结果表明,有机养殖模式下中华绒螯蟹具有较高的抗氧化防护能力和抗菌能力。The activities of lysozyme ,superoxide dismutase （SOD） ,catalase （CAT ） ,and glutathione peroxidase （GSH‐Px） ,and contents of malondialdehyde （MDA） were studied in gill ,hepatopancreas and muscle of Chinese mitten‐handed carb （Eriocheir sinensis） with body weight of 165-175 g farmed under organic and traditional farming patterns . The results showed that the activities of SOD and CAT in hepatopancreas and muscle were significantly higher in the crab farmed in organic pattern than in traditional pattern （P 〈0.05） ,without significant difference in the activity of SOD and CAT in gills （P＆gt;0 .05） .The activity of GSH‐Px was found to be much higher in gill ,hepatopancreas and muscle of Chinese mitten‐handed crab cultured in organic pattern than in traditional pattern （P 〈0 .05） .There was significantly lower content of MDA in gill , hepatopancreas and muscle of Chinese mitten‐handed crab cultured in organic pattern than in traditional pattern （P 〈0 .05） .Lysozyme activitiy was much higher in hepatopancreas of Chinese mitten‐handed crab cultured in organic pattern than in traditional pattern （P 〈0 .05） .The findings indicate that Chinese mitten‐handed crab cultured in the organic pattern show stronger ability of antioxidant defenses and resistance to diseases than the crab farmed in the traditional pattern .

关 键 词：中华绒螯蟹 有机养殖模式 抗氧化酶 丙二醛 溶菌酶 

分 类 号：S968.25[农业科学—水产养殖]