基于血凝素裂解位点的DNA条形码在A型流感病毒分型中的应用  

Application of DNA Barcoding Based on Hemagglutinin Cleavage Site for Influenza A Virus Sub-typing

作　　者：孟慧芝 孙涛[1] 岳志芹[1] 王文斌[2] 

机构地区：[1]山东出入境检验检疫局,山东青岛266000 [2]山西农业大学,山西太谷030801

出　　处：《中国动物检疫》2015年第7期55-61,共7页China Animal Health Inspection

基　　金：国家科技支撑计划(2012BAK11B00)

摘　　要：为了建立一种快速鉴别AIV的HA亚型的RT-PCR方法,本研究探讨了流感病毒血凝素裂解位点基因作为DNA条形码对A型流感病毒进行亚型鉴定的可行性。通过生物信息学方法对16种A型流感病毒的血凝素基因全长进行比对,在HA裂解位点的两端高度保守区设计一对DNA条形码引物,RT-PCR扩增获得约170bp的核苷酸片段。利用MEGA5.0软件构建N-J系统树并计算亚型间及亚型内平均遗传距离。结果显示,不同A型流感病毒裂解位点氨基酸具有单系性,每一亚型可分别形成各自独立的分支。不同亚型间平均遗传距离为0.277,相同亚型内平均遗传距离0.032。研究表明,利用DNA条形码技术可在A型流感病毒分型方面进行有效的分子分类鉴定。The feasibility of the hemagglutinin cleavage sites used as DNA barcoding to subtype influenza A virus was explored in the study. According to the alignment of the hemagglutinin gene of 16 subtypes of influenza A virus by bioinformatics means,a pair of primers were designed on both terminals of highly conserved of hemagglutinin cleavage site.170bp products were obtained by RT-PCR. Then phylogenetic tree was constructed and the average intra-type variation and inter-type divergence were calculated with MEGA5.0 software. The phylogenetic tree revealed that amino acid composition of cleavage site was of monophyly in each subtype. The average genetic distance of inter-type was 0.277,and intra-type genetic distance was 0.032. It was concluded that the hemagglutinin DNA barcoding can be used for subtyping influenza A virus effectively.

关 键 词：A型流感病毒 血凝素(HA) DNA条形码 亚型鉴定 

分 类 号：S852.65[农业科学—基础兽医学]