5种中药对急性感染弓形虫小鼠的治疗效果  

Efficacy of five traditional Chinese medicine against artificial infection of Toxoplasma gondii RH strain in mice

作　　者：张步彩[1,2] 陶建平[1] 蒋春茂[2] 魏宁[2] 王荣林[2] 於敏[2] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏农牧科技职业学院,江苏泰州225300

出　　处：《中国兽医学报》2015年第7期1103-1111,共9页Chinese Journal of Veterinary Science

基　　金：国家"十一五"科技支撑计划重点项目(2010BAD04B02);江苏省高校"动物重要疫病与人兽共患病防控协同创新"项目;第二批泰州市科技发展计划(指导性)项目(2012KJ120)

摘　　要：首先,测定感染小鼠最小致死量;然后,通过体内安全性试验确定各种药物治疗剂量;最后,评价5种中药对急性感染弓形虫小鼠的治疗效果,即:各药物组每只小鼠经腹腔感染25个弓形虫速殖子后4h,小鼠逐只经胃灌服剂量为25g/kg的白牛胆、垂花香薷、黄皮叶或桦褐孔菌,或10.4mg/kg蟾酥、200mg/kg磺胺间甲氧嘧啶钠,每日2次,连用5d,同时设感染不用药组和空白对照组,以小鼠存活率与平均存活时间、腹腔液中虫体数、组织病变程度以及SOD活性等指标评价药物效果。结果显示,磺胺间甲氧嘧啶钠、蟾酥、桦褐孔菌、黄皮叶、白牛胆、垂花香薷组的小鼠存活率分别为66.67%、61.11%、50.00%、5.56%、0%和0%。蟾酥、桦褐孔菌与磺胺间甲氧嘧啶钠组的小鼠平均存活时间、腹腔液中虫体数和血清SOD活性相近,三者间无显著差异(P>0.05),均显著好于黄皮叶、白牛胆、垂花香薷和感染不用药组(P<0.05)。在磺胺间甲氧嘧啶钠组和蟾酥组在肺脏仅出现肺泡隔毛细血管轻微充血,在肝脏和脾脏无明显病变或病变不明显;桦褐孔菌组的肝脏也无明显病变;其他治疗组的脾脏均有不同程度坏死。结果表明,蟾酥与桦褐孔菌的抗弓形虫效果与磺胺间甲氧嘧啶钠相近,白牛胆、垂花香薷和黄皮叶的抗弓形虫效果不明显。In order to screen effective anti-toxoplasmosis drugs,five traditional Chinese medicines were selected to evaluated their efficacy against acute murine toxoplasmosis.Firstly,the minimum lethal dose in mice infected by Toxoplasma gondii was determined.Then,the optimal treatment dose of each drug including sulfamonomethoxine sodium was ascertained in vivo.Finally,efficacies of five traditional Chinese medicines were observed as the following procedures.Each mouse except those in negative control group was intraperitoneally inoculated with 25 tachyzoites of T.gondii RH strain.Four hours later,each mouse except those in negative control group and challenged untreated group were treated with Herba Inulae Cappae（25g/kg）,Elsholtzia penduliflora（25g/kg）,Clausena lansium（Lour.）Skeels leaves（25g/kg）,Inonotus obliquus（25g/kg）,Veneum Bufonis（10.4mg/kg）or sulfamonomethoxine sodium（200mg/kg）by intragastric administration,twice per day for 5days,respectively.The efficacies of drugs were assessed by survival rates and mean survival days of mouse,numbers of tachyzoite in peritoneal exudates,degree of pathological changes of major tissues and activities of SOD in serum.The results showed that survival rates in groups of sulfamonomethoxine sodium,Veneum Bufonis,Inonotus obliquus,Clausena lansium（Lour.）Skeels leaves,Herba Inulae Cappae and Elsholtzia penduliflora were66.67%,61.11%,50.00%,5.56%,0%and 0%,respectively.The mean survival days,numbers of tachyzoites and activities of SOD among the groups of Veneum Bufonis,Inonotus obliquus and sulfamonomethoxine sodium were no significant differences（P〉0.05）,but were significantly different from those in groups of Clausena lansium（Lour.）Skeels leaves,Herba Inulae Cappae,Elsholtzia penduliflora and challenged untreated（P〈0.05）,respectively.In mice treated with sulfamonomethoxine sodium or Veneum Bufonis only slight hyperemia in lung capillaries were observed,and no obvious pathological changes occurred in livers and spleens.There were also no

关 键 词：小鼠 刚地弓形虫 中药 疗效 

分 类 号：S852.7[农业科学—基础兽医学]