分散固相萃取-共振光散射法快速测定蔬果中的毒死蜱  

Rapid dtermination of chlorpyrifos in vegetables and fruit by dispersive solid phase extraction and resonance light scattering method

作　　者：张传林[1] 胡庆红[1] 江波[1] 袁泽利[1] 

机构地区：[1]遵义医学院药学院,贵州遵义563003

出　　处：《化学研究与应用》2015年第7期1092-1096,共5页Chemical Research and Application

基　　金：贵州省科学技术基金项目[黔科合J字LKZ(2011)44]资助

摘　　要：在p H为10.50的Britton-Robinson缓冲液中,毒死蜱与孔雀石绿相互作用,使体系共振光散射强度显著增强,在364 nm、468 nm和726 nm处出现三个特征散射峰,468 nm处为最强散射峰。实验研究了毒死蜱与孔雀石绿相互作用产生的共振光散射光谱,优化了反应条件,毒死蜱浓度在0.1-4.0μg·m L^-1范围内与468 nm处的散射强度有良好的线性关系,其检出限为0.054μg·m L^-1。样品经乙腈提取,中性氧化铝分散固相萃取净化后,各样品中毒死蜱的回收率为89.2%-97.7%。该方法简单、快速、灵敏,可用于蔬果中毒死蜱的检测。In a Britton-Robinson buffer medium（ p H 10. 50）,the resonance light scattering intensity of chlorpyrifos was greatly enhanced when malachite green was added. The three scattering peaks were located at 364 nm,468 nm and 726 nm,with the strongestΔIRLSof 468 nm. The spectra of resonance light scattering,which was caused by the interaction between chlorpyrifors and malachite green had been investigated,and the reaction conditions were optimized. The resonance light scattering intensity was linear to the concentration of chlorpyrifors in the range of 0. 1 - 4. 0 μg·m L^-1at 468 nm with a detection limit of 0. 054 μg·m L^-1. Meanwhile,the samples were extracted by acetonitrile,and purified by dispersion solid phase extraction using neutral alumina. The average recoveries of chlorpyrifors in these samples ranged from 89. 2%to 97. 7%. This method was simple,quick and sensitive for determination of chlorpyrifos residues in the vegetables and fruit.

关 键 词：共振光散射 毒死蜱 孔雀石绿 分散固相萃取 

分 类 号：O657.39[理学—分析化学]