黄瓜和黑籽南瓜幼苗RBCs对低温胁迫的应答差异  

Different responses of root border cells in cucumber and figleaf gourd seedings to low temperature

作　　者：乔永旭[1] 

机构地区：[1]唐山师范学院生命科学系,河北唐山063000

出　　处：《中国农业大学学报》2015年第4期107-112,共6页Journal of China Agricultural University

基　　金：河北省科技计划项目(13226922);河北省高等学校科学技术研究青年基金(QN20132018);唐山师范学院博士基金项目(2014A04)

摘　　要：为探索低温胁迫对黄瓜和黑籽南瓜根系边缘细胞(Root border cells,RBCs)生理特性的影响,以2种瓜类作物的幼苗为试材,研究25和15℃对二者RBCs的数量、活率、黏胶层厚度、凋亡率及根系活力的影响。结果表明:15℃胁迫下,黄瓜RBCs数目和黏胶层厚度的增加程度低于黑籽南瓜,RBCs凋亡率则明显高于黑籽南瓜。此外低温也降低了二者RBCs的活性,黄瓜RBCs活性降低的更明显。RBCs附着根尖时,15℃处理的黄瓜的根系活力明显降低,为对照的28.9%;黑籽南瓜的根系活力变化不明显。去除RBCs后,15℃处理的黄瓜和黑籽南瓜根系活力分别下降了42.6%和27.5%,且均较25℃时差异显著。总之,RBCs能增强植物抵御低温的能力,黑籽南瓜RBCs抵御低温胁迫的能力强于黄瓜。This paper is concerned on the effect of low temperature stress on biological of root border cells（RBCs）from cucumber and figleaf gourd seedlings.The numbers of RBCs,viability of RBCs,thickness of mucilage layer of RBCs,the apoptosis rate of RBCs and root activities were investigated in both species seedlings under 15 and 25 ℃.The results showed that the numbers of RBCs and a thicker mucilage layer of RBCs in figleaf gourd were higher than those in cucumber seedlings.The same trend was observed for apoptosis rate of RBCs and a lower viability of RBCs in cucumber.Root activities under 15 ℃ decreased by 28.9% in cucumber seedlings,but no obvious changes in root activities in figleaf gourd seedlings.Removal of RBCs from root tips led to more severe decline of root activities induced by low temperature in both cucumber and figleaf gourd seedlings.It was concluded that the function of RBCs in protecting root from chilling injury in figleaf gourd seedlings was more important than that in cucumber seedlings.

关 键 词：黄瓜 黑籽南瓜 低温 根系边缘细胞 胁迫 

分 类 号：S567[农业科学—中草药栽培]