MSPD-GC-MS/MS法测定竹笋中26种有机氯农药残留量  

Determination of 26 organochlorine pesticide residues in bamboo shoots by GC-MS/MS coupled with MSPD

作　　者：席慧[1] 杨振兴[1,2] 张帆[1,3] 

机构地区：[1]长沙环境保护职业技术学院,湖南长沙410004 [2]湖南农业大学食品学院,湖南长沙410004 [3]湖南省检验检疫科学技术研究院,湖南长沙410004

出　　处：《湖南林业科技》2015年第4期36-42,共7页Hunan Forestry Science & Technology

基　　金："十二五"国家科技支撑计划(2012BAK08B01)

摘　　要：以基质固相分散-气相色谱-串联质谱法（先将粉粹的竹笋样品经弗罗里硅土基质固相分散技术净化,然后用20 m L丙酮—正己烷溶液（1＋2,v/v）淋洗,GC—MS/MS检测,外标法定量）检测竹笋中26种有机氯农药残留量。结果表明：26种有机氯农药在5~200μg/m L范围内线性良好,相关系数均大于0.99。在0.01~0.1 mg/kg浓度范围内,26种目标物的平均加标回收率为81.1%~108.9%,相对标准偏差在3.3%~9.5%。该方法具有较好的准确度和精密度,各项技术指标均能满足竹笋中26种有机氯农药的检测需要。Developed a method for determination of 26 organochlorine pesticides in bamboo shoots by gas chromatography-tandem mass spectrometry（ GC-MS / MS） using matrix solid phase dispersion clean up. The splintered sample were purified by MSPD using florisil powder and eluted by 20 m L acetone-n-hexane（ 1 ＋ 2,v / v）,the 26 target organochlorine pesticides were rapidly detected by GC-MS / MS using external standard method. The results indicated that the calibration curves of 26 organochlorine pesticides showed good linear relationship in the concentration of 5 ~ 200 μg / m L with correlation coefficients greater than 0. 99. The recoveries of the 26 organochlorine pesticides in bamboo shoot（ from 0. 01 to0. 1 mg / kg） ranged from 81. 1% to 108. 9% with the relative standard deviation（ RSD） ranged from 3. 3% to 9. 5%.It indicated that the method had good accuracy and precision,which can meet the pesticides testing requirements.

关 键 词：竹笋 有机氯农药 基质固相分散 气相色谱—串联质谱 

分 类 号：O652.62[理学—分析化学] S481.8[理学—化学]