地中海蓝钟花(Scilla peruviana)的叶片胚状体诱导及不定芽发生  

Embryoid Induction and Adventitious Buds of Scilla peruviana

作　　者：屈云慧[1] 杨春梅[1] 吴丽芳[1] 单芹丽[1] 

机构地区：[1]云南省农业科学院花卉研究所,云南昆明650205

出　　处：《安徽农业科学》2015年第22期15-16,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立地中海蓝钟花(Scilla peruviana)的离体快繁体系。[方法]以地中海蓝钟花的叶片为外植体进行离体培养,研究不同培养基对叶片诱导胚状体及不定芽发生的影响。[结果]适于地中海蓝钟花叶片愈伤组织诱导及胚状体发生的培养基为MS+6-BA 1.0mg/L+NAA 0.1 mg/L,不定芽分化培养基为MS+6-BA 0.5 mg/L+NAA 0.5 mg/L。[结论]该研究为地中海蓝钟花的快速稳定繁殖提供较好的技术支持。[Objective] The aim was to establish in vitro rapid system for Scilla peruviana. [Method] Using leaves of Scilia Peruriana as explants,the effect of different culture medium on embryoid induction and adventitious buds of Scilla peruviana was studied. [Result] The results showed that MS ＋ 6-BA 1. 0 mg / L ＋ NAA 0. 1 mg / L culture medium was suitable for embryoid induction and adventitious buds of Scilla peruviana; MS ＋ 6-BA 0. 5 mg / L ＋ NAA 0. 5 mg / L was suitable for differentiation of adventitious bud. [Conclusion] The study provided technical support for quickly stable reproduction of Scilla peruviana.

关 键 词：地中海蓝钟花 胚状体 快繁体系 

分 类 号：S188[农业科学—农业基础科学]