阿司匹林预处理对豚鼠内耳缺血再灌注损伤后细胞凋亡的影响  

Effects of Aspirin Pretreatment on Cell Apoptosis in Cochlear of Guinea Pigs after Ischemia-reperfusion Injury

作　　者：许险艳 王庆林 李德水 谢永华 林文东 

机构地区：[1]泉州医学高等专科学校,泉州362000

出　　处：《四川解剖学杂志》2015年第2期12-14,19,共4页Sichuan Journal of Anatomy

基　　金：泉州市科技计划项目(2012Z74)

摘　　要：目的研究阿司匹林(aspirin,ASA)预处理对豚鼠内耳缺血再灌注损伤(IRI)后的保护作用和保护机制。方法健康豚鼠64只,随机分为正常组、假手术组、模型组和干预组(术前腹腔注射阿司匹林50mg/kg,每天一次共7d),模型组和干预组各分成3个亚组:IRI 6h组、24h组和48h组。用HE染色法观察各组耳蜗的形态学改变;用原位凋亡法(TUNEL法)检测耳蜗各部位细胞凋亡情况。结果正常组、假手术组耳蜗罕见凋亡细胞;模型组Corti器、螺旋神经节在再灌注各个时间点均有细胞凋亡,与正常组比较差异有统计学意义(P<0.05);干预组Corti器、螺旋神经节在再灌注各个时间点均有细胞凋亡,程度较模型组减弱,与模型组比较差异有统计学意义(P<0.05)。结论 ASA预处理可能通过抑制细胞凋亡而对缺血再灌注损伤的内耳起保护作用。Objective This study aimed to investigate the effects of aspirin pretreatment on the cell apoptosis in co- chlear of guinea pigs after ischemia reperfusion injury. Methods A total of 64 healthly guinea pigs were devided into the normal group,negative control group, model group and drug pretreated group given aspirin everyday by intraperitoneal injection for 7 days before onset of ischemia. Animals of the last two groups were taken samples at 6 hours,24 hours and 48 hours after reperfusion. The morphologic change of cochlear was observed by HE staining. The cell apoptosis was studied by TUNEL. Results There was no cell apoptosis was found in the normal group and the control group. However, obvious cell apoptosis presented in model groups. The number of apoptotic cells in the drug groups were lesser than that of model groups. Conclusion Aspirin pretreament could inhibit cell apoptosis,which may be one of mechanisms of neuroprotective effects of aspirin.

关 键 词：耳蜗 阿司匹林 缺血再灌注损伤 细胞凋亡 

分 类 号：R764.3[医药卫生—耳鼻咽喉科]