广西地区人群CD36基因rs17154181和rs1761667位点多态性与2型糖尿病大血管病变的关系  

Relationship Between the Polymorphism of rs17154181 and rs1761667 Sites of CD36 Gene and Macroangiopathy in Patients With Type 2 Diabetes Mellitus in Guangxi Province

作　　者：袁雁[1] 庞翠军[1] 肖常青[1] 潘海林[1] 宋琳[1] 廖碧芝 张峥嵘[1] 

机构地区：[1]广西医科大学第一附属医院内分泌代谢科,广西南宁市530021

出　　处：《中国全科医学》2015年第20期2421-2425,共5页Chinese General Practice

基　　金：广西自然科学基金资助项目(2011GXNSFA018257);广西中医药管理局基金项目(GZKZ10-113)

摘　　要：目的探讨CD36基因rs17154181、rs1761667位点多态性与2型糖尿病患者发生大血管病变的关系。方法选取2012年12月—2014年6月于广西医科大学第一附属医院内分泌代谢科住院治疗的2型糖尿病患者112例为病例组,并选取同期于本院体检健康者65例为对照组。记录两组一般资料,并检测相关生化指标。提取外周血DNA,CD36基因rs17154181和rs1761667经PCR扩增后分析基因型。结果两组CD36基因rs17154181、rs1761667位点基因型和等位基因频率比较,差异无统计学意义(P>0.05)。病例组不同程度糖尿病大血管病变患者年龄、总胆固醇(TC)、三酰甘油(TG)水平比较,差异有统计学意义(P<0.05);其中,临床动脉粥样硬化和亚临床动脉粥样硬化患者年龄大于无大血管病变患者,TC水平低于无大血管病变患者;亚临床动脉粥样硬化患者TG水平低于无大血管病变患者(P<0.05)。病例组不同程度糖尿病大血管病变患者CD36基因rs17154181、rs1761667位点基因型及等位基因频率比较,差异均无统计学意义(P>0.05)。病例组rs17154181位点不同基因型患者收缩压比较,差异有统计学意义(P<0.05),其中,基因型AG患者收缩压高于基因型AA患者(P<0.05)。多因素Logistic回归分析显示,年龄、合并高血压、低密度脂蛋白胆固醇(LDL-C)进入回归方程,是2型糖尿病患者发生糖尿病大血管病变的影响因素(P<0.05),而rs17154181、rs1761667位点基因型未进入回归方程(P>0.05)。结论本研究未发现CD36基因rs17154181、rs1761667位点多态性与广西地区2型糖尿病患者发生大血管病变有关,其多态性可能不是CD36与糖脂代谢有关的功能性多态位点。Objective To investigate the relationship between the polymorphism of rs17154181 and rs1761667 sites of CD36 gene and macroangiopathy in patients with type 2 diabetes mellitus in Guangxi Province.Methods We enrolled 112 patients with type 2 diabetes mellitus who received hospitalized treatment in the Department of Endocrinology and Metabolism of the First Affiliated Hospital of Guangxi Medical University from December 2012 to June 2014 as the case group.Another 65 healthy people who underwent physical examination in the same period were enrolled as control group.General data were recorded,and relevant biochemical indicators were tested.DNA in peripheral blood was extracted,and through PCR amplification of rs17154181 and rs1761667 sites of CD36 gene,genotypes were determined.Results The two groups were not significantly different（P 0.05） in the distribution of genotypes and allele frequency at rs17154181 and rs1761667 sites of CD36 gene.In the case group,the diabetic patients with different levels of macroangiopathy were significantly different（P 0.05） in age,TC and TG; patients with clinical arteriosclerosis and subclinical arteriosclerosis were higher（P 0.05） in age and were lower（P 0.05） in TC level than the diabetic patients without macroangiopathy; diabetic patients with subclinical arteriosclerosis were lower（P 0.05） than the diabetic patients without macroangiopathy in TG level.In the case group,the diabetic patients with different levels of macroangiopathy were not significantly different（P 0.05） in the rs 17154181 and rs1761667 genotypes of CD36 genes and allele frequency.In the case group,patients with different genotypes of rs17154181 site were significantly different（P 0.05） in systolic pressure; patients with AG genotype were higher（P 0.05） than patients with AA genotype in systolic pressure.The multivariate logistic regression analysis showed that age,complicated hypertension and LDL-C entered the regression equation,which indicated that these factors were the influenc

关 键 词：糖尿病 2型 糖尿病血管病变 CD36 多态性 单核苷酸 

分 类 号：R587.23[医药卫生—内分泌]