蜗牛多肽对大鼠皮肤烫伤创面的愈合及碱性成纤维生长因子mRNA表达的影响  

Effect of snail polypeptide on skin healing and expression of basic fibroblast growth factor mRNA in rats with scalded wound

作　　者：曹鹏涛[1] 李世朋[2] 张露露[1] 张培哲[1] 袁晓晗[1] 安君岭[1] 席守民[1] 

机构地区：[1]河南科技大学医学院,河南洛阳471003 [2]天津医科大学一中心临床学院,天津300192

出　　处：《中国临床药理学杂志》2015年第14期1426-1428,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家级大学生创新创业训练计划基金资助项目(201210464021);河南科技大学大学生研究训练计划基金资助项目(2013202)

摘　　要：目的探讨蜗牛多肽(SP)对大鼠皮肤烫伤的创面愈合及碱性成纤维生长因子(b FGF)mRNA表达的影响。方法 60只大鼠用90℃热水建立深Ⅱ度烫伤模型,建模后随机分为模型组和实验组,实验组大鼠用SP溶液(10 mg·m L-1)涂抹烫伤部位,模型组大鼠皮肤烫伤处涂抹等量0.9%Na Cl。观察各组大鼠的皮肤愈合率、肤愈时间及脱痂时间,RT-PCR检测皮肤组织中b FGF mRNA表达。结果实验组的烫伤面积愈合率在用药后不同时段均明显高于模型组(P<0.05,P<0.01);与模型组比较,实验组烫伤皮肤的愈合时间与脱痂时间均明显缩短(P<0.05);同时,SP增加了不同时间b FGF mRNA的表达。结论 SP可促进大鼠皮肤烫伤创面愈合,其作用机制可能与调节b FGF mRNA表达有关。Objective To investigate effect of snail polypeptide on skin healing and expression of basic fibroblast growth factor（ b FGF） mRNA in rats with scalded wound. Methods The deepⅡ scald model was established in 60 rats by using 90 ℃ hot water. And the rats were randomly divided into model group and the test group. Rat burn sites in test group were smeared with SP solution（ 10 mg·m L-1）. Skin healing rate,skin healing time and the scab off time were observed in each group of rats.The mRNA expression of skin tissue b FGF was detected by RT-PCR.Results Healing rate of test group at different times after treatment were significantly higher than the model group,with statistically significant difference（ P〈0. 05 or P〈0. 01）. Compared with the model group,burn the skin healing time scab off times are significantly shorter in test group,the difference was statistically significant（ P〈0. 05）. Meanwhile,SP increased the expression of b FGF mRNA at different times.Conclusion SP can promote wound healing in rat skin burns,and its mechanism may be related to regulating b FGF mRNA expression.

关 键 词：蜗牛多肽 烫伤 愈合 碱性成纤维生长因子 

分 类 号：R965.1[医药卫生—药理学]