窄谱中波紫外线联合他克莫司对白癜风模型表皮中黑色素表达的影响  

Influence of combination of narrow band ultraviolet B and tacrolimus ointment on skin melanin expression in models with vitiligo

作　　者：焦亚宁[1] 姜敏[2] 李军晖[3] 刘晓帅[3] 鈡振东 夏莉[1] 梁莉[4] 汪京峡[1] 

机构地区：[1]宁夏医科大学总医院皮肤科,银川750004 [2]宁夏医科大学总医院暨生物芯片北京国家工程研究中心宁夏分中心,银川750004 [3]四川省医学科学院、四川省人民医院实验动物研究所,成都610212 [4]宁夏医科大学研究生学院,银川750004

出　　处：《中国临床药理学杂志》2015年第14期1429-1431,共3页The Chinese Journal of Clinical Pharmacology

基　　金：宁夏自然科学基金资助项目(NZ13151)

摘　　要：目的探讨窄谱中波紫外线(NB-UVB)联合外用他克莫司对白癜风豚鼠模型给药前后组织病理中黑色素数量的变化。方法构建白癜风动物模型,按体重随机分成6组,每组8只,正常组、模型组、对照组(白斑区,外用凡士林,2次/天,连用2个月)、单用药物组(白斑区,外用0.03%他克莫司,2次/天,连用2个月);单用光照组(白斑区,单用NB-UVB照射,隔日1次,连续2个月);联合组(外用他克莫司联合NB-UVB照射,给药频次、方法及时间同单用药物组及单用光照组)。2个月后,通过组织病理学检查,比较给药前后豚鼠表皮组织中黑色素数量的差异。结果与模型组比较,联合组的表皮中黑色素的表达明显增多(P<0.01)。结论联合应用NB-UVB与他克莫司软膏治疗白癜风效果显著。Objective To investigate the changes in the number of skin melanin after combined treatment of narrow band ultraviolet B（ NB-UVB） and tacrolimus ointment in animal models with vitiligo.Methods Animal models with vitiligo were built first. And then all the animal models were randomly divided into six groups of eight according to the principle of weight balance： normal group,model group,control group（ where vaseline was used in the shed white spot areas in the back of animal model,these two methods were implemented 2 times per day）,medication alone group（ 0. 03% tacrolimus ointment skin smear,these two methods were implemented 2 times per day）,light alone group（ white spot area use NB-UVB irradiation,these two methods were irradiation once every other day）, combination group（ topical tacrolimus combination NB-UVB irradiation,frequency,method and time same to medication alone group and light alone group）. The differences of the number of melanin in the skin tissue before and after medication were checked and compared and irradiation by histopathology. Results The effect of the combined group on skin melanin express was significantly different compared with model group（ P〈0. 01）. Conclusion Combination of NB-UVB and tacrolimus ointment has a significant effect on vitiligo treatment.

关 键 词：白癜风 他克莫司软膏 窄谱中波紫外线 黑色素 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]