人参皂苷Rg1对D-半乳糖诱导的亚急性衰老小鼠的肾保护机制研究  

Renal protection of Ginsenosides Rg1 and its mechanism in D-galactose-induced subacute aging mice

作　　者：叶洪[1] 陈晓春 张静 

机构地区：[1]福建医科大学附属协和医院肾内科,福州350001 [2]福建省老年医学研究所,福州350001

出　　处：《中国临床药理学杂志》2015年第14期1435-1438,共4页The Chinese Journal of Clinical Pharmacology

基　　金：福建医科大学青年教师科研基金资助项目(FJGXQ04018)

摘　　要：目的探讨人参皂苷Rg1对D-半乳糖诱导的亚急性衰老小鼠的肾保护机制。方法清洁级昆明小鼠36只按体重随机分为正常组、模型组和实验组,3组均12只。模型组和实验组,给予腹腔注射D-半乳糖诱导小鼠亚急性衰老模型,实验组同时注射人参皂苷Rg1 20 mg·(kg·d)-1,正常组注射等量0.9%Na Cl,连续12周。按试剂盒方法检测丙二醛(MDA)含量、超氧化歧化酶(SOD)含量、脂褐素含量,免疫组化法检测转化生长因子-β1(TGFβ1)和纤连蛋白(FN)在肾组织的表达,Western blot检测TGFβ1表达。结果实验组MDA、脂褐素、TGFβ1、FN的含量均显著低于模型组(P<0.05);实验组SOD活性显著高于模型组(P<0.05)。结论人参皂苷Rg1对D-半乳糖诱导的亚急性衰老小鼠肾具有一定的保护作用。Objective To observe the renal protection of Ginsenoside Rg1 in D-galactose-induced subacute aging mice and to investigate the mecheanism. Methods The 36 Kunming mice of clean grade were randomly divided into three groups,normal group（ n = 12）,model group（ n = 12） and experimental group（ n = 12）. Model group and the experimental group were given intraperitoneal injection of D-galactose in order to make subacute aging mice,the experimental group injected with Ginsenosides Rg1 20 mg ·（ kg · d）-1,for 12 weeks. The normal group was injected with normal saline. The contents of malondialdehyde（ MDA）,superoxide dismutase（ SOD）,and lipofuscin in renal tissue were measured after 12 weeks by Kit. The expression and distribution of transforming growth factor-β1（ TGFβ1） and fibronectin（ FN） in renal tissue were detected by immunohistochemical assay. And the level of TGFβ1 was investigated by Western-blot. Results Lipofuscin and MDA in the model group were markedly increased while the activities of SOD decreased significantly in comparison with those in the control group（ P〈0. 05）. The lipofuscin and MDA decreased significantly in the experimental group（ P〈0. 05）,the activities of SOD were higher than those in the model group,and has significant difference（ P〈0. 05）.There were no obvious difference of lipofuscin,MDA and SOD activity between the experimental group and the normal group. Compared withthe control group,the expression of TGFβ1,FN in model group was significantly increased（ P〈0. 05）. The expression of TGFβ1,FN in the experimental group was significantly lower than those in the model group（ P〈0. 05）. The renal expression of TGFβ1 was increased remarkably in model group,and was reduced significantly in the experimental group（ P〈0. 05）. Conclusion Ginsenoside Rg1 has protective effect on D-galactose-induced subacute aging mice.

关 键 词：人参皂苷RG1 D-半乳糖 亚急性衰老小鼠 肾 保护机制 

分 类 号：R965.1[医药卫生—药理学]