高效液相色谱测定比格犬血浆中的头孢唑啉与头孢曲松的浓度  

Determination of cefazolin and ceftriaxone in Beagle dog plasma by high performance liquid chromatography

作　　者：李天云[1] 

机构地区：[1]北京大学第一医院临床药理研究所,北京100034

出　　处：《中国临床药理学杂志》2015年第14期1452-1454,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的建立HPLC在多项测定参数相同的基础上,分别测定比格犬血浆中头孢唑啉、头孢曲松浓度的方法。方法色谱柱：头孢唑啉为ES industries Epic C18（4.6 mm×150 mm,5μm）,头孢曲松为Phenomenex Gemini C18（4.6 mm×150mm,5μm）,流动相：头孢唑啉为甲醇-0.05 mol·L-1乙酸铵缓冲液（p H=4.3）=30∶70;头孢曲松为乙腈-0.05 mol·L-1KH2PO4缓冲液（p H=6.0）=6∶94,流速均为1.0 m L·min-1,进样量均为40μL。结果头孢唑啉标准曲线回归方程为y=-1.25×104x＋8479.37（r=0.997 4）,头孢曲松为y=1.25×104x＋2.72×104（r=0.997 6）。头孢唑啉线性范围为2-300μg·m L-1,头孢曲松为2-200μg·m L-1;日内、日间精密度相对标准偏差均≤10%,提取回收率均≥85.78%。结论本法灵敏、准确、快速,可用于比格犬血浆头孢唑啉、头孢曲松浓度的测定和药物其他方面研究。Objective To develop a HPLC method for the determination of cefazolin and ceftriaxone in vivo. Methods Ceftriaxone use Phenomenex Gemini C18（ 4. 6 mm × 150 mm,5 μm） and cefazolin use ES industries Epic C18（ 4. 6 mm × 150 mm,5 μm） columns,all were used at 30℃. Cefazolin with a mobile phase of（ p H = 4. 3） 0. 05 mol·L-1ammonium acetate-methanol solution（ 70 ∶ 30）,ceftriaxone with a mobile phase of（ p H = 6. 0） 0. 05 mol · L-1phosphater buffer-acetonitrile（ 94∶ 6）. All at a flow-rate of 1. 0 m L·min-1. Injection volume of 40μL all were used. Quantitaion was performed using HPLC. Results The straight line corresponding to equation were obtained： y =-1. 25 × 104 x ＋8479. 37（ r = 0. 997 4） in the range of 2-300 μg · m L-1for cefazolin;and y = 1. 25 × 104 x ＋ 2. 72 × 104（ r = 0. 997 6） in the range of 2-200μg · m L-1for ceftriaxone. The extraction recovery for cefazolin and ceftriaxone were ≥ 85. 78%. The intra and inter day precision were ≤10%. Conclusion The analytical method appeared to be accurate,sensitive and convenient for the determineation of cefazolin and ceftriaxone in Beagle dog. It is suitable for animal pharmacokinetic study of cefazolin and ceftriaxone or in other fields.

关 键 词：高效液相色谱法 头孢唑啉 头孢曲松 比格犬 血浆 

分 类 号：R969.1[医药卫生—药理学]