IL-27在Graves病患者外周血中的表达  

Expression of IL-27 in peripheral blood of patients with Graves' disease

作　　者：姚秋明[1] 王媛[1] 周娇珍 蔡甜甜[1] 徐健[1] 张健[2] 张进安[1] 

机构地区：[1]复旦大学附属金山医院内分泌科,上海201508 [2]复旦大学附属金山医院检验科,上海201508

出　　处：《现代免疫学》2015年第4期299-301,共3页Current Immunology

基　　金：国家自然科学基金(81270871);上海市卫计委科研基金(2012-225)

摘　　要：为了探讨IL-27与Graves病发病的关系检测了Graves患者外周血单个核细胞中IL-27p28/EBI3mRNA的表达和外周血血清中IL-27的水平。收集Graves患者及健康对照者抗凝外周静脉血,分离外周血单个核细胞(peripheral blood mononuclear cells,PBMC),采用实时定量PCR(real-time PCR)技术,检测IL-27p28/EBI3mRNA的表达;通过收集Graves患者及正常对照者外周静脉血,采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血清中IL-27的水平。结果显示:1、与对照组相比,IL-27p28/EBI3mRNA在Graves患者外周血PBMC中的表达升高(P<0.05)。2、Graves患者血清中IL-27水平较健康对照者升高(P<0.05)。3、血清中IL-27水平与促甲状腺素受体抗体滴度之间无相关性(P>0.05)。因此,我们推测IL-27参与了Graves的发病,未来仍需进一步的研究阐明IL-27在Graves中发挥的具体作用。To evaluate the relationship between IL-27 and Graves＇ disease （GD）,we determined the expression of IL-27p28/ EBI3mRNA in the peripheral blood and IL-27 level in the serum of patients with Graves＇disease. Peripheral venous blood sam- ples were collected from GD patients and healthy controls, then peripheral blood mononuclear cells （PBMCs） and serum were i- solated. We used Real-time PCR to detect the level of IL-27p28/EBI3mRNA. The concentration of IL-27 in the serum was de- termined by ELISA. The results showed that the mRNA expressions of subunits of IL-27p28 and EBI3 in GD patients were higher than those of the normal controls （P〈0.05, both）, serous IL-27 levels were higher in GD patients compared with the normal controls（P〈0.05） , but no correlation was found between serum IL-27 and TRAb. So we conclude that interleukin-27 may implicated in the pathogenesis of Graves＇disease, which has been speculated as a therapeutic potential in GD, though further investigations are needed to establish the precise role of IL-27 in GD.

关 键 词：GRAVES病 白介素-27 细胞因子 

分 类 号：R392.11[医药卫生—免疫学]