Luminex液相芯片检测系统性红斑狼疮患者外周血IFN-γ、IL-4和IL-17水平  

Detection of serum IFN-γ,IL-4 and IL-17 in systemic lupus erythematosus using a new liquid chip based on Luminex XMAP

作　　者：马小美[1] 陈金烟 余莲[1] 

机构地区：[1]福建医科大学附属龙岩第一医院血液风湿科,龙岩364000 [2]福建省医学研究院,福州350000

出　　处：《现代免疫学》2015年第4期311-315,共5页Current Immunology

摘　　要：运用Luminex液相芯片检测SLE患者外周血IFN-γ、IL-4和IL-17水平变化与疾病的关系。选择82例SLE患者,其中30例SLE为初治患者,采用Luminex液相芯片检测82例SLE患者、30例初治患者治疗后三个月时血清中IFN-γ、IL-4和IL-17水平,通过横向研究82例SLE患者其血清水平变化及与SLEDAI相关性,纵向研究30例SLE患者初治时、治疗后三个月时其血清水平变化及意义。结果显示,IL-17在SLE患者活动组显著高于稳定组、健康对照组,稳定组显著高于健康对照组(P<0.05),IFN-γ、IL-4、IFN-γ/IL-4水平在SLE患者活动组、稳定组、健康对照组三组之间差异无统计学意义;IL-17在SLE患者初治组、治疗后3个月组明显高于健康对照组,初治组明显高于SLE治疗后3个月组(P<0.05),IFN-γ、IL-4、IFN-γ/IL-4水平在SLE患者初治组、治疗后3个月组、健康对照组三组之间无统计学意义;IL-17与SLEDAI呈正相关,(r=0.338);IFN-γ与IL-17正相关性(r=0.364),血清IL-17水平在有肾脏损害的SLE患者中表达增高,差异具有显著的统计学意义(P<0.05)。研究表明,SLE患者外周血存在IFN-γ、IL-4、IL-17表达的失平衡,即CD4+T辅助细胞亚群失平衡,参与了疾病的发生过程和发展。Abstract： To detect serum levels of IFN-γ, IL-17 and IL-4 in systemic lupus erythematosus （SLE） using the Luminex Liquid chip , and to explore the clinical significance of these cytokines for SLE, 82 SLE patients and 30 healthy controls were enrolled in this study. Among the patients, 30 cases were initially diagnosed and had never received any treatment before the start of the study. The serum levels of IFN-γ, IL-4, IL-17 were measured by Luminex liquidchip. The 82 patients with SLE were cross- sectionally analyzed for the relationship between the disease and the 3 cytokines, SLEDAI as well as clinical manifestations. In addition, a longitudinal study was carried out for the 30 cases to compare the levels of the 3 cytokines before and after treatment for 3 months. The results showed that The serum level of IL-17 in active SLE patients was significantly higher than that in the inactive patients and the healthy controls（P〈0.05）. The serum level of IL-17 in the initially diagnosed, both before treatment and after 3 month＇ treatment was significantly higher than that of the healthy controls. For the initially group, after 3 months＇ treatment, IL-17 level was significantly decreased than before treatment. （P〈0.05）. As to levels of IFN-γ,IL-4 and the ratio of IFN-γ/IL-4, no significant differences were found between the SLE patients and the controls. There was a positive correla- tion between serum level of IL-17 and SLEDAI in SLE patients（r=0. 338）, and a positive correlation between serum levels of IFN-γ and IL-17 in SLE patients（r=0. 364）. The serum level of IL-17 in SLE patients with renal damage was significantly increased as compared with those with normal renal functions（P〈0.05）. Taken together, we conclude that a balance between Th1 ,Th2 and Th17 cells is disrupted in SLE patients, and may he involved in the pathogenesis of SLE.

关 键 词：系统性红斑狼疮 T辅助细胞 细胞因子 

分 类 号：R593.241[医药卫生—内科学]