中药白疕合剂对体外培养HaCaT细胞肿瘤坏死因子-α、白细胞介素-6表达的影响  

Effects of White Mange Mixture on the Expression of Tumor Necrosis Factor-α,Interleukin-6 in Cultured HaCaT Cells

作　　者：陈兴[1] 卢益萍[1] 李忻红[1] 

机构地区：[1]辽宁中医药大学附属医院,沈阳110032

出　　处：《中国中西医结合皮肤性病学杂志》2015年第3期151-153,158,共4页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

基　　金：辽宁省自然科学基金 项目编号:201102149

摘　　要：目的观察中药白疕合剂对体外培养人永生化表皮角质形成细胞(Ha Ca T细胞)分泌肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6及TNF-αm RNA、IL-6 m RNA表达的影响,并探讨其作用机制。方法采用ELISA法检测白疕合剂对Ha Ca T细胞分泌TNF-α、IL-6的影响;采用RT-PCR方法检测白疕合剂对Ha Ca T细胞TNF-αm RNA、IL-6m RNA表达的影响。结果白疕合剂低、中、高剂量组干预Ha Ca T细胞后,对TNF-α、IL-6的分泌量及TNF-αm RNA、IL-6 m RNA的表达均有明显的抑制作用。白疕合剂高剂量组对IL-6分泌量的抑制作用及降低TNF-αm RNA、IL-6m RNA的表达与消银颗粒组和阿维A组比较差异无统计学意义;对TNF-α的抑制作用与消银颗粒组比较差异有统计学意义,与阿维A组比较差异无统计学意义。结论白疕合剂可能通过抑制TNF-α、IL-6的分泌和降低TNF-αm RNA、IL-6 m RNA的表达,发挥对银屑病的治疗作用。Objective To observe the effects of white mange mixture on the level of TNF-α, IL-6 and the expression of TNF-αm RNA, IL-6 m RNA in cultured Ha Ca T cells and to explore its mechanism. Methods ELISA was applied to test the effects of white mange mixture on the level of TNF-α and IL-6. The expression of TNF-α m RNA and IL-6 m RNA were detected by RTPCR. Results White mange mixture（ low, middle and high dose group） intervening Ha Ca T cells could obviously inhabited the level of TNF-α, IL-6 and decreased the expression of TNF-α m RNA, IL-6 m RNA. White mange mixture in the high dose group had no significant difference with Xiaoyin granules group and acitretin group in inhibiting the content of IL-6 and decreasing the expression of TNF-α m RNA, IL-6 m RNA. Inhibition of TNF-α had significant differences comparing with Xiaoyin granules group, and no significant differences comparing with acitretin group. Conclusion White mange mixture plays a role in the treatment of psoriasis possibly through the inhibition of the content of TNF-α, IL-6 and decreasing the expression of TNF-αm RNA, IL-6 m RNA.

关 键 词：白疕合剂 人永生化表皮角质形成细胞 肿瘤坏死因子-Α 白细胞介素-6 肿瘤坏死因子-αm RNA 白细胞介素-6 M RNA 

分 类 号：R285.5[医药卫生—中药学]