甘蓝型油菜矮秆突变基因ndf1的分子标记连锁遗传作图  

Genetic mapping of the dwarf mutant gene ndf1 in Brassica napus L.

作　　者：杨朋娜[1] 张璐[1] 史慧娟[1] 李清[1] 王茂林[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2015年第4期889-894,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家"863"计划(2011AA10A10401);四川省十二五油菜育种攻关项目(2011yzgg05);国家科技支撑计划(2013BAD01B03)

摘　　要：利用240对SSR和672对SRAP分子标记,以甘蓝型油菜矮秆突变株系与高秆野生型杂交的回交一代(BC1)群体为材料,对甘蓝型油菜矮秆突变基因ndf1进行连锁遗传作图分析.结果表明:(1)在240对SSR引物中,有145对的扩增产物显示出突变株系与野生型间具有多态性,采用矮秆与高秆杂交F2群体集团分离分析法(BSA)筛选连锁分子标记,并利用BC1群体进行遗传连锁作图分析,发现位于13号连锁群上的Na12-E02和CB100572对SSR标记与矮秆突变基因ndf1位点紧密连锁,2对标记位于矮秆突变基因ndf1的同侧,连锁图距分别为6.34cM、8.77cM;(2)经过672对SRAP分子标记连锁遗传分析,获得了4对与矮秆突变位点连锁的SRAP标记:Me15em4-288、Me28em3-171、Me25em15-262和Me3em18-684,与矮秆突变位点的连锁图距分别为12.48cM、15.19cM、20.19cM和35.46cM,Me28em3-171和Me3em18-684位于矮秆突变基因ndf1的另一侧.240 pairs of SSR and 672 pairs of SRAP molecular markers were employed for the dwarf mu- tant gene ndfl mapping in a BC1 population derived from backcross between the dwarf mutant and the wild type inbred line. Results showed that： （1） 145 of 240 SSR primers showed polymorphisms between the dwarf mutant and the wild type. Then we used bulked segregation analysis （BSA） to select markers in the Fz dwarf （D） pool, high （H） pool, and used BCI individuals to make the genetic linkage analy- sis. 2 of 145 SSR primers, namely Nal2-E02 and CB10057 on N13, were linked to the gene ndfl at a distance of 6. 34cM and 8. 77cM respectively. Both of them were on the same side of ndfl. （2） The re- sults of 672 pairs of SRAP markers polymorphic analysis revealed that 4 SRAP primers, MelSem4-288, Me28em3-171, Me25em15-262 and Me3em18-684 were linked to the mutant gene ndfl at a distance of 12. 48cM, 15. 19cM, 20. 19cM and 35. 46cM respectively. Me28em3-171 and Me3em18-684 were on the other side of gene ndfl.

关 键 词：甘蓝型油菜 矮秆突变体 分子标记 连锁作图 

分 类 号：Q37[生物学—遗传学]