微生物电解乙酸合成高值有机物  

Study of high value organics synthesis from acetate reduction in microbial electrochemical systems

作　　者：王娟[1,2] 朱晓宇[2] 刘柯[2] 李建[1,2] 李大平[2] 高平[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]中国科学院成都生物研究所,成都610041

出　　处：《四川大学学报（自然科学版）》2015年第4期925-931,共7页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31270166)

摘　　要：以微生物电解池为平台,考察了阴极电势,pH以及初始乙酸盐浓度对乙酸还原及其丁酸、乙醇等产物积累的影响.结果显示,恒定阴极电势-900mV(vs.Ag/AgCl)、pH6.0时,初始乙酸钠浓度为1.0g/L时,丁酸产量最高,可达到80(±5)mg/L,乙醇积累26(±5)mg/L.在-900mV阴极电势下,总碳回收率达到63.2%,产物电子回收率为13.06%;在-800mV阴极电势下,总碳回收率达到42.8%,产物电子回收率为54.4%.控制阴极电势-850mV,初始乙酸钠浓度由1g/L增加到5g/L,丁酸的产量由48.8mg/L增加到76.2mg/L,提高了56%.扫描电镜显示,阴极碳毡上菌体主要为杆菌.对阴极附着微生物进行变性梯度凝胶电泳(PCR-DGGE)分析发现,假单胞菌属(Pseudomonas)和梭菌属(Clostridium)为主要的功能菌群,假单胞菌可能与阴极电子传递有关,而梭菌可能与高值有机物的合成有关.The effect of cathode, pH and initial concentration ot acetate on ttae acetate reaucuon ano me accumulation of butyrate, ethanol and other products were studied taking microbial electrochemical tech- nology as a platform. In the batch test of biocathode potential, the highest production rate of butyrate and ethanol was 80 （ 4± 5） mg/L and 26 （ ±5） mg/L （at potential-900 mV）. Electron and carbon bal- ances revealed that the cathodic carbon efficiency and electron efficiency was 63.2% and 13.06% at po- tential of -900 inV. At potential of -800 mV, the cathodic carbon efficiency and electron efficiency was 42.8% and 54.4%, respectively. Batch test of pH indicated that pH6.0 was the best condition of pro- ducing high value organics. At the potential of -850 mV, the yield of butyrate increased from 48. 8mg/ L to 76.2mg/L, the concentration of butyrate increased by 56% from I g/L to 5 g/L sodium acetate. SEM showed the cathodes mainly included rod-shaped organism. PCR-DGGE revealed that Pseudomonas and Clostridium were dominant populations. transfer, and clostridium may be associated Pseudomonas may be associated with the cathode electron with the synthesis of high value organics.

关 键 词：微生物电化学技术 生物阴极 乙酸 丁酸 乙醇 

分 类 号：Q93[生物学—微生物学]