透骨消痛胶囊干预兔膝骨关节炎蛋白多糖与MMP-2、MMP-13表达的实验研究  

Experimental Study on Tougu Xiaotong Capsule (透骨消痛胶囊) Interfering in the Expression of Proteoglycan,MMP-2 and MMP-13 in Rabbits with Knee Osteoarthritis

作　　者：陈赛楠[1] 黄云梅 陈文列[1,2] 黄美雅[1] 吴广文[1] 林煜[3] 林如辉 李西海 于超[1] 冯芳芳[1] 李孝栋 刘献祥[1] 

机构地区：[1]福建中医药大学中西医结合研究院,福建福州350122 [2]国家中医药三级科研实验室-中药药理(细胞结构与功能)实验室,福建福州350108 [3]厦门大学附属福州第二医院,福建福州350007 [4]福建省中西医结合老年性疾病重点实验室,福建福州350108

出　　处：《风湿病与关节炎》2015年第7期5-10,60,共7页Rheumatism and Arthritis

基　　金：国家自然科学基金(81202712);福建省自然科学基金(2014J01356);福建省科技重大项目(2012Y4006)

摘　　要：目的:通过透骨消痛胶囊对兔膝骨关节炎软骨基质多糖与基质金属蛋白酶(MMP)-2、MMP-13表达影响的实验研究,进一步探讨该药保护骨关节炎软骨基质的作用机制。方法:将72只新西兰大白兔随机分为空白对照组,模型对照组,奥泰灵组,透骨消痛胶囊低、中、高剂量组,每组12只。除空白对照组外,其余各组均采用改良Hulth法造模,造模后5周给予药物治疗,灌胃8周。甲苯胺蓝染色、AB-PAS染色观察软骨蛋白多糖的表达,Western Blot检测MMP-2、MMP-13的表达。结果:与空白对照组比较,模型对照组甲苯胺蓝染色、AB-PAS染色显著减弱,MMP-2、MMP-13表达显著升高。与模型对照组比较,透骨消痛胶囊组甲苯胺蓝染色、AB-PAS染色均匀深染,MMP-2、MMP-13表达显著降低。结论:透骨消痛胶囊可通过下调软骨MMP-2、MMP-13表达,减缓软骨基质降解,降低软骨损伤程度,延缓骨关节炎病理进程。Objective：By study on Tougu Xiaotong Capsule（透骨消痛胶囊） interfering in the expression of proteoglycan,MMP-2 and MMP-13 in rabbits with knee osteoarthritis to further explore the action mechanism of the drug to protect cartilage matrix.Methods：72 New Zealand rabbits were randomly divided into a blank control group,a model control group,an Aotailing（Glucosamine Hydrochloride Capsules） group,and Tougu Xiaotong Capsule groups（low,medium and high dose groups）.Except for the blank control group,models were established in the other groups with modified Hulth method,and treated with drugs after 5 weeks—given intragastric administration for 8 weeks.Toluidine blue staining and AB-PAS staining were used to observe the expression of cartilage proteoglycan,and Western Blot was used to detect the expression of MMP-2 and MMP-13.Results：Compared with the blank control group,Toluidine blue staining and AB-PAS staining in the model control group were significantly weakened,while the expression of MMP-2 and MMP-13 significantly increased.Compared with the model control group,Toluidine blue staining and AB-PAS staining in the Tougu Xiaotong Capsule groups were homogeneously hyperchromatic and the expressions of MMP-2 and MMP-13 were significantly decreased.Conclusion：By the down regulation of the expressions of MMP-2 and MMP-13,Tougu Xiaotong Capsule can slow down the degradation of cartilage matrix,reduce the severity of cartilage injury,and delay the pathological process of osteoarthritis.

关 键 词：骨关节炎 膝 透骨消痛胶囊 蛋白多糖 MMP-2 MMP-13 

分 类 号：R285[医药卫生—中药学]