香蕉MaPIP2-2基因的克隆、亚细胞定位及表达分析  

Cloning, Subcellular Localization and Expression Analysis of MaPIP2-2 Gene in Banana

作　　者：侯晓婉 胡伟[2] 颜彦[2] 徐碧玉[2] 金志强[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《热带作物学报》2015年第7期1267-1273,共7页Chinese Journal of Tropical Crops

基　　金：现代农业产业技术体系(国家香蕉产业技术体系)建设专项资金资助(No.CARS-32);973计划前期研究专项(No.2014CB160314)

摘　　要：水通道蛋白(AQPs)是细胞间和细胞内水分运输的主要通道,对于植物细胞的水分稳态和胁迫响应具有重要作用。本研究从香蕉中克隆了一个水通道蛋白基因Ma PIP2-2。序列分析结果表明,该基因的开放阅读框(ORF)为846 bp,编码281个氨基酸。多序列比对和进化树分析结果表明,该基因编码的蛋白与其它植物中水通道蛋白具有较高的一致性,并且与拟南芥At PIP2-2和At PIP2-3的亲缘关系最近。亚细胞定位表明该基因定位在细胞膜上。实时荧光定量PCR分析结果表明,该基因能够在转录水平响应甘露醇、低温、Na Cl胁迫和ABA信号。这些结果表明Ma PIP2-2可能参与了非生物逆境胁迫应答,为进一步研究Ma PIP2-2基因的功能鉴定了基础。Water channel protein, （Aquaporins, AQPs）, which was the main water transport corridor, plays a major role in maintaining water steady state and the stress response. In the present study, a A QP gene designated MaPIP2-2 from banana was isolated. MaPIP2-20RF was 846 bp, encoding 281 amino acids. Comparison of amino acid sequences and phylogenetie analysis indicated that MaPIP2-2 was closely related with AtPIP2-2 and AtPIP2- 3. Subeellular localization assays showed that the MaPIP2-2 protein was present in the plasma membrane. Real- time quantitative polymerase chain reaction （QPCR） assay revealed that MaPIP2-2 participated in the response of abiotic stresses at transcriptional level. These results indicated that MaPIP2-2 might be involved in abiotie stress responses. The study would lay a foundation for further investigating the function of MaPIP2-2.

关 键 词：水通道蛋白 PIP 香蕉 克隆 亚细胞定位 荧光定量PCR 

分 类 号：Q344.13[生物学—遗传学]