铜绿金龟子肠道链霉菌Streptomyces sp.BCa1菌体的抗菌成分研究  

Antibacterial Metabolites from the Mycelia of the Cockchafer-Derived Streptomyces sp. BCa1

作　　者：郭志凯[1] 刘寿柏[1] 马帅[1] 王蓉 

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101 [2]海南省海洋与渔业科学院,海南海口570203

出　　处：《热带作物学报》2015年第7期1307-1311,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.41406083);海南省自然科学基金(No.313079;314176)

摘　　要：为从昆虫来源的放线菌次级代谢产物中寻找药物先导分子,对铜绿金龟子幼虫肠道链霉菌Streptomyces sp.BCa1菌丝体化学成分进行了研究。通过活性跟踪,从其菌丝体的氯仿甲醇萃取物中分离得到主要成分1。利用高分辨质谱、一维和二维核磁波谱技术,将该主要成分1鉴定为具有双重旋转对称结构的不饱和大环内酯类抗生素阿扎霉素(elaiophylin),具有显著的抗金黄色葡萄球菌活性。该类化合物为首次从昆虫来源的放线菌中发现。In order to find drug leads from insect-associated actinomycetes, guided by antibacterial activity assay, a major bioactive constituent 1 was isolated from the organic solvent （chloroform and methanol mixture） extract of the myceha of the cockchafer （Anomala corpulenta Motsch） -derived Streptomyces sp. BCal. The compound was identified as elaiophylin （1）, a symmetrical macrolide antibiotic with 16-membered unsaturated dilactone ring, on the basis of spectroscopic analyses including HR-ESI-MS, 1D and 2D NMR experiments. Compound 1 showed significant antibacterial activity against Staphylococcus aureus. This kind of compound was firstly reported from an insect-derived Streptomyces.

关 键 词：铜绿金龟子 链霉菌 菌体 阿扎霉素 抗菌活性 

分 类 号：O629.9[理学—有机化学]