从噬菌体随机七肽库中筛选抗H3N2亚型犬流感病毒多肽的研究  

A study of anti-H3N2 canine influenza virus polypeptides selected from the phage display random heptapeptide library

作　　者：叶存栋 胡静思[1,2] 贾坤[1,2] 李陆涛[1,2] 刘荣昌[1] 涂黎晴[1,2] 孙凌霜[1,2] 李守军[1,2] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]华南农业大学广东省兽医临床重大疾病综合防控重点实验室,广东广州510642

出　　处：《华南农业大学学报》2015年第5期12-17,共6页Journal of South China Agricultural University

基　　金：国家自然科学基金(31372448);公益性行业(农业)科研专项(201303042);国家重点基础研究发展计划(2011CB504700-G);广东省兽医临床重大疾病综合防控重点实验室资助项目(2013A061401013);广东省自然科学基金(S2013020013858);广东省科技计划项目(2011B020306007);促进科技服务业发展计划项目(2013B040200032)

摘　　要：【目的】以纯化的H3N2亚型犬流感病毒HA1蛋白为作用靶点,从噬菌体随机七肽库中筛选出具有抗流感病毒活性的亲和多肽.【方法】运用噬菌体展示技术,以纯化的H3N2亚型犬流感病毒HA1蛋白为作用靶点,从噬菌体随机七肽库中筛选HA1蛋白亲和多肽,并对获得的多肽进行鸡胚水平和细胞水平抗H3N2亚型流感病毒活性验证.【结果和结论】经过4轮体外亲和筛选获得了6条HA1蛋白亲和多肽,6条多肽对H3N2亚型流感病毒有不同程度的抗病毒活性,其中以HA-4的抗病毒活性最强.试验结果表明噬菌体随机肽库技术能够应用于抗病毒研究.【Objective】To find specific affinity peptide which inhibited replication of canine influenza virus( CIV),and the ligands on the CIV Hemagglutinin HA1 subunits were screened from the phage display random library. 【Method】The CIV Hemagglutinin HA1 subunits as the target were screened out through affinity selection from the heptapeptide phage library,and the affinity peptide which possessed anti-viral properties of H3N2 subtype of influenza virus were tested in chicken embryos or vitro.【Result and conclusion】After four rounds of affinity panning,six peptides were identified. Six peptides have various degrees of an antiviral activity of H3N2 subtype in vitro or vivo,especially the peptide HA-4,which shows the strongest antiviral activity. Experimental results show that the phage random peptide library technology can be used in the antiviral research.

关 键 词：犬流感病毒 H3N2亚型 血凝素HA1亲和肽 噬菌体随机肽库 抗病毒多肽 

分 类 号：S859[农业科学—临床兽医学]