甜玉米类胡萝卜素合成关键基因PSY1、LCYE和CrtRB1的功能分析  

Functional analysis of the key carotenoid biosynthetic genes PSY1,LCYE and CrtRB1 in sweet corn

作　　者：冯发强[1] 王国华[1] 王青峰[1] 杨瑞春[1] 李小琴[1,2] 

机构地区：[1]华南农业大学农学院/广东省植物分子育种重点实验室,广东广州510642 [2]华南农业大学亚热带农业生物资源保护与利用国家重点实验室,广东广州510642

出　　处：《华南农业大学学报》2015年第5期36-42,共7页Journal of South China Agricultural University

基　　金：国家自然科学基金(31071427);高等学校博士学科点专项科研基金(20114404110005)

摘　　要：【目的】鉴定玉米类胡萝卜素合成关键基因PSY1、LCYE和CrtRB1的功能标记在47份甜玉米骨干自交系中的多态性和对类胡萝卜素各组分含量的影响,为了解玉米类胡萝卜素合成关键基因的功能及维生素A源强化育种提供参考和依据.【方法】以47份甜玉米骨干自交系为材料,用高效液相色谱法检测籽粒乳熟期类胡萝卜素各组分的含量,合成基因PSY1、LCYE和CrtRB1的6个功能标记并在47份甜玉米自交系中检测其基因型,结合基因型和类胡萝卜素各组分的含量,检测3个关键基因的单倍型效应,并分析联合单倍型效应.【结果和结论】除CrtRB1基因的标记Indel4未检出多态性,其余标记均检测出多态性.PSY1的Indel1和Indel4组成的单倍型可解释玉米黄质和总类胡萝卜素含量表型变异的14.81%和13.00%,LCYE的5'Indel和3'Indel位点分别可解释β-胡萝卜素、维生素A源和总类胡萝卜素含量表型变异的15.77%、20.75%和15.92%,CrtRB1的标记3'TE未检测出显著性.基因PSY1、LCYE和CrtRB1组成的联合单倍型分别可解释β-胡萝卜素、维生素A源和总类胡萝卜素含量表型变异的37.20%、40.71%和41.11%.基因间联合单倍型效应高于单基因单倍型效应.PSY1和LCYE有利等位基因对甜玉米中类胡萝卜素的合成有重要影响,其功能标记可用于分子标记辅助选择,为甜玉米的维生素A源强化育种奠定了基础.【Objective】To identify the polymorphisms of functional markers for the key carotenoids biosynthetic genes PSY1,LCYE and CrtRB1 in sweet corn and their effects on the contents of carotenoid for a better understanding of the functions of these genes so as to provide a reference and basis for bio-fortification breeding of pro-vitamin A. 【Method 】The contents of carotenoid components were measured by HPLC in 47 sweet corn elite inbred lines. Six functional markers for PSY1,LCYE and CrtRB1 genes were synthesized and amplified to detect the genotypes of these lines. The effects of single gene haplotype andjoint haplotype were analyzed using the functional polymorphic sites of PSY1,LCYE,and CrtRB1 genes together with the carotenoid content variation.【Result and conclusion】The polymorphism was detected in5 functional markers except for indel4 for CrtRB1. The haplotype with Indel1 and Indel4 for PSY1 could explain 14. 81% of the zeaxanthin content variation and 13. 00% of the total carotenoid content variation.The haplotype with 5'Indel and 3'Indel for LCYE could explain 15. 77% of β-carotene content variation,20. 75% of pro-vitamin A content variation and 15. 92% of the total carotenoids content variation. No significant effect was detected in the haplotype of CrtRB1. The joint haplotype of PSY1,LCYE and CrtRB1 could explain 37. 20% of β-carotene content variation,40. 71% of pro-vitamin A content variation and41. 11% of the total carotenoids content variation. The joint effect of PSY1,LCYE and CrtRB1 was higher than that of single gene. PSY1 and LCYE genes are important in the biosynthesis of carotenoid. Their functional markers can be used in the molecular-assisted selection and provide a theoretical basis for biofortification of pro-vitamin A in sweet corn by breeding.

关 键 词：甜玉米 PSY1基因 LCYE基因 CrtRB1基因 类胡萝卜素 单倍型 

分 类 号：S513[农业科学—作物学]