温哥华假单胞菌菌株PAN4解磷能力及对核桃的促生作用  

The phosphate-solubilizing ability and growth promoting effects of Pseudomonas vancouverensis strain PAN4 on walnut

作　　者：谯天敏[1] 李姝江[1] 韩珊[1] 朱天辉[1] 王树山[1] 

机构地区：[1]四川农业大学林学院,四川成都611130

出　　处：《华南农业大学学报》2015年第5期117-124,共8页Journal of South China Agricultural University

基　　金：国家自然科技资源共享平台(2005DKA21207-13)

摘　　要：【目的】探讨温哥华假单胞菌Pseudomonas vancouverensis菌株PAN4解磷能力的动态变化及不同培养条件对菌株解磷能力的影响,及其对核桃Juglans sigllata苗的促生作用和光合特性的影响.【方法】利用Pikovskaya培养液[液体蒙金娜培养基(PVK)]研究温哥华假单胞菌溶磷特性,单因素试验测定碳源、氮源、初始培养液pH、温度对其解磷能力的影响,采用盆栽试验探究在灭菌土和未灭菌土中菌剂对核桃苗的促生效应.【结果和结论】随时间延长,温哥华假单胞菌菌株PAN4培养的离心上清液中,有效磷含量显著增加,48 h时达到峰值;麦芽糖、玉米浆分别为最适碳源、氮源;pH7、温度28℃时,菌体生长量最大,解磷能力最强,二者呈显著正相关关系.不同浓度菌剂均可使核桃苗的株高、地径、净光合速率、呼吸速率和叶绿素含量不同程度地增加,浓度越高增幅越大;施复合肥的效果不如108和109cfu·m L-1的菌剂;菌剂在非灭菌土中的促生效果比灭菌土好.【Objective】The dynamic change of phosphate-solubilizing ability of Pseudomonas vancouverensis strain PAN4 under different culture conditions and the effects of growth promotion and the photosynthetic characteristics of walnut,Juglans sigllata,seedlings were studied. 【Method】The phosphate-solubilizing property was studied in liquid Pikovskaya medium( PVK). The influences of carbon source,nitrogen source,initial p H and temperature on phosphate-solubilizing of P. vancouverensis strain PAN4 were determined using a single factor test. The growth promoting effects on walnut seedlings were conducted in sterilized soil and unsterilized soil,respectively. 【Result and conclusion】The available phosphorus content in supernatant of strain PAN4 significantly increased with time extending,with the maxium content of available phosphoru reaching a peak at 48 h. The malt and corn steep liquor was the optimal carbon source and nitrogen source,respectively. The maxium growth mass and the maxium phosphate-solubilizing ability were obtained at 28 ℃ under pH 7,and both had a significantly positive correlation. The increase of plant height,basal diameter,net photosynthetic rate,transpiration rate and chlorophyll content correlated withthe concentrations: the higher the concentration,the more increases. The effect of compound fertilizer was less than those of the bacterial inoculant with the concentrations of 10~8 and 10~9cfu · m L^(-1). The growth promoting effect of the bacterial inoculant was better in unsterilized soil than that in sterilized soil.

关 键 词：温哥华假单胞菌 解磷能力 核桃 促生作用 光合特性 

分 类 号：S154.3[农业科学—土壤学] S664.1[农业科学—农业基础科学]