氨溴索对胃内容物吸入性肺损伤JNK信号通路的影响  

Effect of ambroxol inhalation lung injury of JNK signaling pathway in gastric aspiration lung injur

作　　者：张衍民[1] 贾晓民[1] 赵杰[1] 李海泉[1] 马雷[1] 徐俊马[1] 杭文璐[1] 

机构地区：[1]徐州医学院第二附属医院呼吸内科,江苏徐州221006

出　　处：《河北联合大学学报（医学版）》2015年第4期4-7,共4页Journal of North China Coal Medical College

摘　　要：①目的探讨氨溴索对大鼠胃内容物吸入后损伤肺组织中c-氨基末端蛋白激酶(c-jun N-terminal kinases,JNK)信号通路的影响。②方法 40只健康Sprague-Dawley雄性大鼠随机分为4组:对照组、损伤组、SP600125(JNK特异性阻断剂)组、氨溴索组,每组10只;复制大鼠胃内容物吸入性肺损伤动物模型。检测各组大鼠肺泡灌洗液(BALF)中性粒细胞计数与丙二醛(MDA)活性、肺组织湿重/干重比(W/D)、髓过氧化物酶(Myeloperoxidase,MPO)活性变化。Western-blot方法检测肺组织中JNK、磷酸化JNK(p-JNK)及诱导型一氧化氮合酶(i NOS)的蛋白表达;光镜下观察肺组织结构变化。③结果与对照组比较,损伤组BALF中性粒细胞计数与MDA活性、肺组织湿重/干重(W/D)比值以及MPO活性增加(均P<0.05);p-JNK与i NOS蛋白表达均显著增高(均P<0.05);肺组织出现明显病理组织学损伤。与损伤组比较,SP600125组和氨溴索组BALF中性粒细胞计数与MDA活性、肺组织W/D比值以及MPO活性下降,差异有统计学意义(均P<0.05);p-JNK与iNOS蛋白表达均显著下降(均P<0.05);肺组织病理学损伤减轻。Western-blot结果显示各组大鼠肺组织中JNK蛋白表达无差异。④结论 JNK参与胃内容物吸入性肺损伤炎症反应,氨溴索可能通过抑制JNK信号通路、抑制iNOS表达发挥抗炎、抗氧化保护作用。Objective To investigate effect of ambroxol hydrochloride inhalation lung injury in tissue of c-N-terminal protein kinase signal pathway in Gastric Aspiration lung Injury. Methods 40 healthy male Sprague-Dawley rats were randomly divided into 4 groups： control group,injury group,SP600125（ JNKspecific inhibitor） group,ambroxolgroup,10 rats in each group; rat ofaspiration of gastric contents of lung injuryanimal model was remolded. Bronchoalveolarlavage fluidof rats（ BALF） neutrophil count and malondialdehyde（ MDA）activity,the lung wet weight / dry weight ratio（ W / D）,myeloperoxidase（ Myeloperoxidase,MPOactivity changes.Detection of lung tissue Western-blot method of JNK and p-JNK（ phosphorylated JNK） and inducible nitric oxidesynthase（ i NOS） protein expression; observe the lung tissue structure changesunder light microscope. Results Compared with the control group,injury neutrophil count and MDA ingroup BALF（ MDA） activity of neutral,lung wet weight / dry weight ratio,MPO activity increased（ P〈0. 05） p-JNK and i NOS protein expression were significantly increased（ P 0. 05）; lung tissue appeared obvious histopathological injury. Compared with the injury group,SP600125 group andambroxol group BALF neutrophil count and malondialdehyde（ MDA） activity,lung wetweight / dry weight ratio and the activity of MPO decreased,the difference was statistically significant（ P 0. 05）; p-JNKand i NOS protein expression were significantly decreased（ P 0. 05）; Damage of the lung tissue pathology was reduced. Western-blot results showed that thelung tissue of rats in each group were no difference in the expression of JNK protein. Conclusion JNK is involved in gastric contents inhalation lung injury and inflammation reaction,the protective effect of ambroxol on anti-inflammatory,antioxidant may be related to the inhibition of JNK signaling pathway and the inhibition of i NOS expression.

关 键 词：氨溴索 肺损伤 JNK 

分 类 号：R563[医药卫生—呼吸系统]