紫外线灭活仙台病毒诱导人胶质瘤细胞系LN229凋亡作用(英文)  

Ultraviolet-inactivated Sendai virus strain Tianjin induced apoptosis in human glioma cell line LN229

作　　者：石立莹[1] 李梅[1] 张磊[1] 耿鹏[1] 李咏梅[1] 

机构地区：[1]天津医科大学基础医学院微生物学教研室,天津300070

出　　处：《中国人兽共患病学报》2015年第7期597-601,共5页Chinese Journal of Zoonoses

基　　金：Funded by the National Natural Science Foundation of China(No.81172168)~~

摘　　要：目的探讨紫外线灭活仙台病毒致人胶质瘤LN229细胞凋亡作用及其机制。方法用不同剂量灭活病毒感染LN229细胞,24h后,MTT法检测灭活病毒对细胞增殖的影响;流式细胞仪检测细胞凋亡情况;分光光度法检测caspase活性;Western blotting检测凋亡相关蛋白的表达水平。结果 MTT检测显示灭活仙台病毒能够剂量依赖性抑制LN229细胞增殖;流式细胞仪检测显示灭活病毒组细胞凋亡率呈剂量依赖性升高;caspase活性测定显示灭活病毒组细胞中caspase-3,-8和-9蛋白活性呈剂量依赖性增加;Western blotting结果显示,随着灭活病毒滴度增加,细胞中Bax、细胞色素c、Fas、FasL表达水平升高、而Bcl-2、caspase-8前体、caspase-9前体和caspase-3前体蛋白表达下降。结论灭活仙台病毒能够诱导LN229细胞剂量依赖性凋亡,其机制与线粒体途径和死亡受体途径相关。Sendal virus strain Tianjin was a novel genotype of Sendai virus. This study was designed to investigate the effect of ultraviolet-inactivated, replication-defective Sendal virus strain Tianjin （UV-Tianjin） on apoptosis of human glioma cell line LN229 and the possible mechanism. MTT assay showed that treatments with UV-Tianjin dose-dependently inhibited the prolif- eration of LN229 ceils. Flow cytometric analysis after Annexin V/PI staining showed that UV-Tianjin induced apoptosis of LN229 cells in a dose-dependent manner. Colorimetric detection of caspase activity suggested that the activities of easpase-3, -8 and -9 increased with the increasing of viral titer. Moreover, Western blotting analysis showed that treatments with UV-Tianjin increased the expression levels of Bax, cyt c, Fas, FasL and decreased the expression levels of Bcl-2, pro-caspase-8, pro- easpase-9 and pro-caspase-3 in LN229 cells in a dose-dependent manner. Our study suggests that UV-Tianjin exhibits the anti- cancer activity in human glioma cell line LN229 through inducing apoptosis, which may involve in both the endogenous mito- chondrial pathway and the exogenous death receptor pathway.

关 键 词：仙台病毒 人胶质瘤细胞系LN229 凋亡 线粒体途径 死亡受体途径 

分 类 号：R373.1[医药卫生—病原生物学]