抑制Kupffer细胞功能减轻日本血吸虫病肝脏肉芽肿炎症反应  

Inflammatory response of schistosomiasis hepatic granuloma by inhibiting the function of Kupffer cells

作　　者：杨江华[1] 孙静[1] 梁曼曼[1] 盛浩宇[1] 王文节[1] 

机构地区：[1]皖南医学院弋矶山医院感染性疾病科,芜湖241001

出　　处：《中国人兽共患病学报》2015年第7期623-626,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(81141083);安徽省高校省级自然科学研究重点项目(KJ2014A271)~~

摘　　要：目的通过制备氯磷酸二钠脂质体（LE-Cl2MDP）,选择性剔除日本血吸虫感染小鼠模型中Kupffer细胞,研究抑制Kupffer细胞功能对日本血吸虫肝脏肉芽肿形成的影响。方法采用脂质体包裹CL2MDP;6～8周龄雌性BALB/c小鼠分为3组：PBS组,日本血吸虫感染组,日本血吸虫感染联合LE-Cl2BMP组;对于联合组,小鼠尾静脉注射LE-Cl2MDP（0.1mL/10g）,每周2次,连续6周;感染后第12周,剥杀小鼠,留取血清及肝脏,用于细胞因子和免疫组织化学检测等,数据统计分析。结果经过LE-Cl2MDP处理后,日本血吸虫感染肉芽肿周围Kupffer细胞细胞明显减少;无论是感染第8周,还是第12周肝组织中虫卵肉芽肿周围炎症细胞浸润明显减少,纤维化程度减轻;同时降低感染第12周小鼠血清IL-10水平,LE-Cl2MDP组为（42.6±10.4）pg/mL,感染组为（67.4±12.9）pg/mL（P〈0.05）。结论本研究建立LE-Cl2MDP剔除小鼠肝脏Kupffer细胞的日本血吸虫感染模型,抑制了肝组织中虫卵肉芽肿周围炎症反应。Liposome-encapsulated dichloromethylene diphosphonate （LE-C12 MDP） was prepared to selectively eliminate out Kupffer cells in a mouse model of Schistosoma japonica infection. Further studies by inhibiting the effects of Kupffer cell func- tion were implemented to judge whether it impacted on the liver granuloma formation of Schistosoma japonica infection. Lipo- some was used to package CL2 MDP. The 6-8 weeks old female BALB/c mice were divided into three groups, including phos- phate buffered saline （PBS） group, Schistosoma japonica infection group and Schistosoma japonica infection combined LE-Clz BMP group. The combination group model was reproduced by injection of LE-C12 BMP into tail vein （0.1 mL/10 g） for twice a week through six weeks. Mice were sacrificed at 12 week post infection with Schistosoma japonicum, serum and liver speci- mens were collected to detect eytokines and immunohistoebemistry, etc. Statistical analysis was performed based on relevant data. Results showed that after LE-ClzMDP treatment, Kupffer cells of Schistosoma japonica egg granuloma significantly re- duced. Inflammatory cells infiltration surrounding schistosome egg granuloma in liver also decreased significantly, and that alle- viated hepatic fibrosis at 8 or 12 week. At 12 weeks after infection, the level of IL-10 in the sera of the mice with schistosomia- sis infection combined LE-C12 MDP group （42. 6 10.4） pg/mL was significantly lower than that of schistosomiasis infection group （67.4 12.9） （P〈0.05）. This study established the model that LE-C12MDP was able to eliminate the hepatic Kupffer cells in the mice infected with Schistosoma japonicurn, and it also inhibited inflammatory response around schistosomal egg granuloma.

关 键 词：血吸虫病 KUPFFER细胞 氯磷酸二钠 脂质体 

分 类 号：R383.24[医药卫生—医学寄生虫学]