比较5种混合固定液对小鼠眼球标本制片质量和染色效果的影响  

Effect of 5 mixed stationary liquids on the mouse eyeball specimen sectioning and the staining quality

作　　者：罗灿峤[1] 莫木琼[1] 聂钊铭[1] 钟觉民[1] 李宇彬[1] 

机构地区：[1]中山大学附属第一医院,广东广州510058

出　　处：《中国卫生检验杂志》2015年第13期2065-2067,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学青年基金(1100470;81270750);中山大学青年教师培育项目(12ykpy24);广东省自然科学基金资助项目(S2012010009390)

摘　　要：目的通过小鼠眼球标本的制片质量和染色效果的比较,筛选最佳固定液。方法取健康成年小鼠眼球400只,随机分为5组,分别投入中性甲醛钙液、甲醛-乙醇固定液(Verhoeff液)、重铬酸钾-甲醛-冰醋酸-三氯醋酸-醋酸钠水溶液固定液(Kolmer液)、甲醛-冰醋酸-乙醇固定液(FAA液)、甲醛-冰醋酸-乙醇-生理盐水混合固定液(改良FAA液),按8 h、12 h、24 h及48 h固定,每个时间段取出20只,常规制片、苏木素-伊红(HE)染色,显微镜下观察。结果 5种固定液对小鼠眼球标本都有固定作用。Kolmer液固定效果最差:优级片0张(0%),中级片1张(5%),差级片19张(95%),不适用于眼球标本的固定。改良FAA液固定效果最好:优级片19张(95%),中级片1张(5%),差级片0张(0%),且以固定24 h最佳。结论改良FAA液固定小鼠眼球,标本的制片质量和染色效果最好,适用于临床和科研。Objective Comparing the sectioning and dyeing effects of five different fixing solutions on the normal eyeballs of mice, so as to select the optimum mixed fixing solution. Methods Four hundred eyeballs from healthy adult mice were obtained and randomly divided into 5 groups, which were, respectively, dived into the neutral formaldehyde calcium solution, Verhoeff solution, Kolmer solution, formalin - calcium liquid, FAA liquid group and the modified FAA liquid. After 8 h, 12 h, 24 h and 48 h, respectively, 20 eyeballs were undergone routine sectioning, HE staining and morphology was observed under microscope. Results All the solutions were useful to the fixation of eyeballs. The fixed effect of the Kolmer solution was the worst with top -grade 0（0% ）, serie B piece 1 piece（5% ）, poor C 19（95% ）, which did not apply to eye specimens fixing. The fixing effect of the FAA solution was the best with top -grade 19（95% ）, serie B piece 1 piece（5% ）, poor C 0（0% ） and the best fixing was at 24 h. Conclusion The use of modified FAA mixed liquid on fixing mouse eyeball is the best to sample production and staining quality, and is suitable to be applied in the clinical and scientific research.

关 键 词：固定液 眼球 质量 差异 

分 类 号：R361.2[医药卫生—病理学]