离子液体基金纳米流体修饰电极用于多巴胺的测定  

Ionic lquid-based nanofluids containing gold nanoparticles modified glassy carbon electrode for the determination of dopamine

作　　者：刘洁[1] 丁素芳[1] 徐冉冉[1] 陈礼威[1] 

机构地区：[1]淮北师范大学化学与材料科学学院,安徽淮北235000

出　　处：《中国卫生检验杂志》2015年第13期2080-2082,2086,共4页Chinese Journal of Health Laboratory Technology

基　　金：安徽省高等学校自然科学研究基金资助课题(KJ201-0A305);安徽省科技厅自然科学基金资助课题(11040-606M42);国家级大学生创新创业训练计划基金资助课题(201310373034);安徽省大学生创新创业训练计划项目基金资助课题(201310373054)

摘　　要：目的依据文献先合成离子液体基金纳米流体(ILAu NFs),再采用滴涂法将合成的离子液体基金纳米流体固定在玻碳电极表面,构建测定多巴胺(DA)的电化学传感器。方法采用循环伏安法(CV)研究DA在修饰电极上的电化学行为,以差示脉冲伏安法(DPV)建立测定DA的方法。结果在p H=7.0的磷酸缓冲溶液(PBS)中,ILAu NFs对DA具有明显的电催化作用,DA在修饰电极上呈现一对峰形良好的氧化还原峰;与裸电极相比,DA在修饰电极上的氧化峰电位负移113 m V,峰电位差△Ep由219 m V减小至64 m V,可逆性明显变好,而且峰电流明显增加。在优化的实验条件下,DA氧化峰的电流与其浓度在4.0×10-6mol/L^1.0×10-4mol/L呈良好的线性关系,检出限为1.32×10-7mol/L。结论建立的测定DA的电化学新方法可用于实际样品盐酸多巴胺注射液中DA含量的测定,结果令人满意。Objective A new electrochemical sensor for detecting dopamine （DA） was fabricated by immobilizing the ionic liq- uid -based nanofluids containing gold nanoparticles （ILAuNFs）, which was prepared according to the literature, on a glassy carbon electrode surface by drop - coating method. Methods The cychc vohammetric method was adopted to research the electrochemical behavior of DA on the modified electrode, and the new detection method of DA was developed by differential pulse voltammetry（DPV）. Results In phosphate buffer solution （pH = 7.0）, ILAuNFs had obvious electro -catalysis effect on DA. A pair of well - defined quasi - reversible redox peaks of DA was obtained on the modified electrode. Compared with the bare electrode, the oxidation peak potential of DA shifted negatively 113 mV at the modified electrode and the peak current obviously increased. The peak potentials separation （AEp） decreased from 219 mV to 64 mV. Under the experimental optimized conditions, the DA oxidation peak current demonstrated the good linear relationship with the concentration range of 4.0 × 10 ^-6 - 1.0 × 10 ^-4 mol/L. The detection limit was 1.32 × 10^ -7 mol/L. Conclusion The new electrochemical method can be applied to determination of the content in the actual sample of DA hydrochloride injection with satisfactory results.

关 键 词：多巴胺 离子液体基金纳米流体 电催化 玻碳电极 

分 类 号：O657.1[理学—分析化学]