应用巢式PCR法检测综合医院肺炎患者痰样本中的嗜肺军团菌  

Detection of Legionella pneumophila in sputum of pneumonia patients from general hospital by nested PCR methods

作　　者：张宝莹[1] 白雪涛[1] 刘凡[1] 刘航[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100050

出　　处：《中国卫生检验杂志》2015年第13期2115-2117,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学基金课题(81172626);北京市自然科学基金(7122117);卫生行业科研专项课题(201002001)

摘　　要：目的调查综合医院肺炎患者嗜肺军团菌的感染情况。方法 2012年6月-2013年6月,选择北京市丰台区、江苏省常州市和无锡市、广东省深圳市综合性医院的肺炎住院患者为调查对象,采集肺炎患者的痰样本,问卷调查收集患者的基本信息,采用基于mip基因的巢式PCR法检测肺炎患者痰样本中的嗜肺军团菌。结果共调查157例肺炎住院患者,痰样本嗜肺军团菌的阳性率是12.1%(19/157),北京市丰台区、江苏省无锡市、江苏省常州市和广东省深圳市4地区综合医院肺炎患者痰样本中嗜肺军团菌的阳性率分别为11.9%、11.8%、20.0%和6.5%,差异无统计学意义(χ2=3.404,P=0.333)。入院前没有户外活动和有户外活动的肺炎患者痰样本中,嗜肺军团菌的阳性率分别为9.4%和15.3%,差异无统计学意义(χ2=1.261,P=0.261)。结论综合医院肺炎患者存在嗜肺军团菌感染。Objective To investigate LegioneUa pneumophila infection status of pneumonia patients from general hospital. Methods The sputum was collected from pneumonia patients in general hospital of four cities （Fengtai district of Beijing, Changzhou, Wuxi and Shenzhen） from June 2012 to June 2013. The basic information of pneumonia patient was collected by questionnaires. Legionella pneumophila in sputum was detected by nested PCR based on mip gene. Results The positive rate of Legionella pneumophila in sputum of 157 pneumonia patient from general hospital was 12.1% （ 19/157 ）. The positive rate of LegioneUa pneumophila in sputum of pneumonia patient in Fengtai district of Beijing, Wuxi, Changzhou and Shenzhen were 11.9%, 11.8%, 20.0% and 6.5%, respectively. There was no statistical significance on the differences （X2 = 3. 404, P = 0. 333 ）. The positive rate of Legionella pneumophila in sputum of pneumonia patients with and without doing outdoor activities before admission were 9.4% and 15.3%. There was no statistical significance on the differences （X2 = 1. 261, P = 0. 261 ）. Conclusion There is Legionella pneumophila infection of pneumonia patients from general hospital.

关 键 词：嗜肺军团菌 痰 巢式聚合酶链式反应 肺炎患者 综合医院 

分 类 号：R563.1[医药卫生—呼吸系统]