雌激素对失血性休克孕兔发生急性肺损伤的影响及其机制  

Protective effect of estrogen on acute lung injury after hemorrhagic shock in pregnant rabbit

作　　者：吴焕[1] 龚时鹏[1] 刘士三[1] 姚硕[1] 刘倩倩[1] 余艳红[1] 

机构地区：[1]南方医科大学南方医院妇产科,广州510515

出　　处：《中华妇产科杂志》2015年第7期522-528,共7页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的探讨雌激素对失血性休克孕兔发生急性肺损伤的影响及其机制。方法60只孕兔随机分为6组，每组10只，正常对照组（无任何处理）、雌激素对照组（静脉注射苯甲酸雌二醇0．37mg／kg），以下4组孕兔先行休克及复苏处理后再分组处理：雌激素休克组（静脉注射苯甲酸雌二醇0．37mg／kg）、果糖休克组（静脉注射5％果糖2ml／kg）、p38丝裂原活化蛋白激酶（p38激酶）抑制剂休克组（静脉注射p38激酶抑制剂2mg／kg）、p38激酶抑制剂＋雌激素休克组（处理同p38激酶抑制剂休克组及雌激素休克组）。各组孕兔分别于实验0、60、80及260min时，检测血清肿瘤坏死因子α（TNF-α）及白细胞介素6（IL-6）的水平；孕兔处死后行肺组织丙二醛（MDA）含量、髓过氧化物酶（MPO）及超氧化物歧化酶（SOD）活性的检测；计算肺组织的肺干／湿质量（DW／WW）比值；对肺组织进行病理检查。结果（1）正常对照组及雌激素休克组孕兔血清TNF-α水平在实验0min、60min时与其他4组比较，差异均无统计学意义（P〉0．05）。实验80、260min时，各休克组孕兔血清TNF-α水平呈上升趋势，雌激素休克组[分别为（172．4±16．0）、（216．7±18．6）ng／L]、果糖休克组[分别为（171．6±9．1）、（263．9±7．8）ng／L]、p38激酶抑制剂休克组[分别为（172．8±7．2）、（300．6±4．8）ng／L]及p38激酶抑NN＋雌激素休克组[分别为（167．9±4．8）、（261．8±9．6）ng／L]，分别与正常对照组和雌激素对照组比较，差异均有统计学意义（P〈0．05）。（2）正常对照组及雌激素休克组孕兔血清IL-6水平在实验0、60、80min时分别与其他4组比较，差异均无统计学意义（P〉0．05）；实验260min时，雌激素休克组、果糖休克组、p38激酶抑制剂休克组及p38激酶抑制剂＋雌激素休克组孕兔血清IL-6水平[分别为（98．3±0．9）�Objective The paper is an attentative effort to evaluate the reaction and mechanism of estrogen on pregnant rabbits with acute lung injury caused by hemorrhagic shock. Methods Sixty pregnant New Zealand white rabbits were randomly divided into 6 groups, with 10 rabbits in each group, namely normal control group （NG group, with anesthesia only）, estrogen group （E2G group, with additional estrogen injection at 60 rain） and the other four hemorrhagic shock groups underwent hemorrhagic shock （i.e. E2SG, FSG, SBSG, E2SBSG group; mean blood pressure-40 mmHg（1 mmHg=0.133 kPa） by phlebotomy for 15 rain. After maintenance of the pressure for 45 rain, the rabbits were treated with Eg（0.37 mg/kg）, fructose injection（5%,2 ml/kg）, the p38 mitogen-activated protein kinases（p38MAPK） inhibitor SB-203580 （2 mg/kg） or E2 plus SB-203580. Tumor necrosis factor alpha（TNF-α, interleukin-6（IL-6） were measured at different time points（0min, 60 min, 80 min and 260 min）, lung tissue methane dicarboxylic aldehyde（MDA） level, lung tissue myeloperoxidase（MOP）, superoxide dismutase（SOD） activity, lung tissue dry weight/wet weight （DW/WW） value were measured after the experiment was finished, pulmonary pathology of the rabbits was observed. Result （1） Serum TNF-α level of NG group and E2SG group were not significantly different compared with the other four groups at the 0 min and 60 min. At 80 min and 260 min of experiment, serum TNF-α level of all the four shock groups were increased, E2SG group [（172.4± 16.0） and （216.7± 18.6） ng/L], FSG group [（171.6±9.1） and （263.9±7.8） ng/L], SBSG group [（172.8±7.2） and （300.6±4.8） ng/L], E2SBSG group [（167.9±4.8 ）and （261.8±9.6） ng/L], and significantly higher than NG group and E2G group, separately （P〈0.05）. （2） Serum IL-6 level of NG group and E2SG group were not significantly different compared with the other four groups at the 0 min, 60 min and 80 min. At 260 min, the serum IL-6 level[（9

关 键 词：雌激素类 休克 出血性 急性肺损伤 产后出血 疾病模型 动物 

分 类 号：R714.461[医药卫生—妇产科学]