盐田嗜盐单胞杆菌的分离鉴定及其PHB积累的研究  

Isolation and identification of Halomonas sp. from solar saltpond and study of PHB accumulation in its cells

作　　者：隋丽英[1] 张娇娇[1] 许高超 邓元告[1] 

机构地区：[1]天津科技大学海洋科学与工程学院天津市海洋资源与化学重点实验室,天津300457

出　　处：《海洋科学》2015年第5期16-20,共5页Marine Sciences

基　　金：国家自然科学基金资助项目(31172427);"大学生创新创业训练"计划项目(201310057010)

摘　　要：微生物聚合物聚-？-羟基丁酸酯（PHB）具有良好的生物降解性、生物相容性和热塑性,在环保、医药和农业领域具有广阔的应用前景。高PHB积累菌株的获得及其发酵条件的研究具有很好的实用价值。作者从渤海湾日晒盐场盐田卤水中分离出一株杆状细菌,经16S r DNA序列测序比对,确定为盐杆菌属（Halomonas）,命名为Halomonas 100-16-2,其最适生长盐度为50~100,最适生长p H为7~8,属中度嗜盐菌。在酸水解酪蛋白（7.5 g/L）的基础培养基中分别添加0、10、20和30 g/L葡萄糖,研究盐度100条件下不同葡萄糖添加量对Halomonas 100-16-2生长和PHB含量的影响。结果表明,随着葡萄糖浓度的增加,该菌株的生长和PHB积累显著提高。葡萄糖质量浓度为30 g/L时,细胞干质量达8.60 g/L,PHB含量达到5.57 g/L（即达到细胞干质量的65%）。本研究为渤海湾盐田微生物资源的开发和利用提供了有价值的基础数据。Poly-β-hydroxybutyrate（PHB） is the polyester reserved in cells of microorganisms. It has biodegradable, biocompatible and thermoplastic characteristics, thus has a great potential of commercial application in the field of environmental protection, medicine and agriculture. It is important to obtain high-PHB accumulated bacteria and study their fermentation condition. A rod bacterial strain was isolated from brine water in saltpond of Bohai Bay coastal solar saltworks. Phylogenetic analysis of the 16 S r RNA gene sequence indicated that the isolate was Halomonas sp., namely Halomonas 100-16-2. It is a moderately halophilic bacterium with optimal growth salinity of 50-100 and pH of 7-8, respectively. The effect of increasing glucose supplementation（at level of 0, 10, 20 and 30 g/L, respectively） on growth and PHB content of Halomonas 100-16-2. was investigated using basal culture medium containing 7.5 g/L acid-hydrolyzed casein. The results showed that glucose supplementation in the culture medium significantly improved the growth and PHB content of Halomonas sp. At a glucose concentration of 30 g/L, the cell dry weight and PHB content reached 8.60 g/L and 5.57 g/L（65% of cell dry weight）. The current research has provided valuable data with respect of utilization of brine water bio-resources.

关 键 词：嗜盐细菌 盐杆菌属(Halomonas) PHB积累 葡萄糖 

分 类 号：Q939.97[生物学—微生物学]