生物甲烷气实验的外加菌源(沼液)中厌氧菌群测定  

Determination of Anaerobic Bacterial Consortia in Added Microbial Sources(Biogas Slurry) for Biogenic Methane Experiment

作　　者：夏大平[1,2] 王振[1] 苏现波[1,3] 司青[1] 

机构地区：[1]河南理工大学 能源科学与工程学院,焦作454000 [2]中原经济区煤层(页岩)气河南省协同创新中心,焦作454000 [3]河南省瓦斯地质与瓦斯治理重点实验室-省部共建国家重点实验室培育基地,焦作454000

出　　处：《高校地质学报》2015年第1期168-171,共4页Geological Journal of China Universities

基　　金：国家自然科学基金(41472127;41472129);河南省教育厅科学技术研究重点项目(13A440335;132102210253);山西省煤层气联合研究基金资助项目(2013012004);河南省高校科技创新团队支持计划(15IRTSTHN027)联合资助

摘　　要：考虑到煤本源微生物的分离、纯化及培养相对困难,且耗时较长,采用沼液作为外加菌源,并在进行生物甲烷气实验之前,对所采集沼液中的主要厌氧菌群进行微生物特征及数量的测定。结果表明沼液中的厌氧发酵菌(Anaerobicfermentative bacteria)、厌氧纤维素分解菌(Anaerobic cellulose-decomposing bacteria)、硫酸盐还原菌(Sulfate-reducingbacteria)和产甲烷菌(Methanogens)4类厌氧菌群,均具有杆状、球状及链状等多种形态;染色后发现其中革兰氏阴性菌占主要优势;经最大或然数(MPN)计数后表明,沼液中所含厌氧菌的数量足以进行后续的生物甲烷气的产出实验。Given that indigenous microbial consortia of raw coal samples are in great difficulty being separated, purificated andcultured, which are invariably at a big cost of time, biogas slurry was considered to be added as the microbial sources, of which themorphological characteristics and the amount of dominant anaerobic bacteria were determined prior to the conduction ofbiogenic-methane-production experiment. The results show that four types of anaerobic microbial consortia including fermentativebacteria, cellulolytic bacteria, sulfate-reducing bacteria and methanogens were separately indentified and observed with suchrecognizable morphological features as rod-shaped, spherical and streptobacteria; the gram-negative bacteria were found to be at anotable advantage after gram stain; further the content of microbial consortia determined by most probable number（MPN） countingmethod was deemed to be enough for following biogenic-methane-production experiment.

关 键 词：本源微生物 沼液 生物甲烷气 厌氧菌群 MPN计数 

分 类 号：Q93-33[生物学—微生物学]