新生大鼠HIBD后海马p-Tau蛋白与脑神经元凋亡表达变化  

The Expression Changes of Microtubule-associated Protein p-tau and neurone Apoptosis in the Hypoxic-ischemic Neonatal Rats

作　　者：袁俊英[1] 张冬秀[1] 张利红[1] 王军[1] 

机构地区：[1]郑州大学第三附属医院脑康中心,河南郑州450000

出　　处：《中国伤残医学》2015年第15期15-18,共4页Chinese Journal of Trauma and Disability Medicine

摘　　要：目的：观察微管相关蛋白磷酸Tau（ p-tau）在缺氧缺血性脑损伤（ HIBD）新生大鼠脑组织中的表达变化. 方法：将80只7日龄SD清洁级大鼠随机分为假手术组40 只和HIBD组（均按处死时间点分为3、6、12、24、48 小时5 个亚组）40只. 参照Rice法建立HIBD动物模型,假手术组仅暴露左颈总动脉,不作缺氧处理,2 组均于HIBD手术结束后3、6、12、24、48小时5个时间点断头取脑. 并分别取其脑组织切片,采用免疫组化S-P法检测其脑室周围白质区 p-tau 的表达,末端脱氧核苷酸转移酶缺口标志 （ TUNEL） 法检测其神经细胞凋亡. 结果：假手术组海马区有部分 p-tau蛋白表达及少量凋亡细胞, 各时间点无明显变化. HIBD组 p-tau的表达在3 小时后开始增加,12 小时达表达高峰后即开始呈下降趋势,与假手术组比较差异均有统计学意义 （F=95.67,p=0.000 〈0.05） ;细胞凋亡在处理后3 小时即开始增加,至24小时小时达表达高峰,与假手术组比较差异均有统计学意义（F=18.829,p=0.001 〈0.05）. 结论：HIBD可造成新生大鼠海马区p-tau蛋白及神经元凋亡表达增加.Objecit ve：Observing the expression changes of p -tau protein , and neurone apoptosis in hypoxic-ischemic brain damage rats, and exploring their correlations.Method s：Seven-day-old newborn clean Sprague Dawley（ SD） rats 80 , were randomly divided into sham operation group and HIBD group, each one got 40.HIBD group established animal model by Rice method, Sham group just ex-posed the left carotid artery, two groups were both decapitated and took the brain at 3h、6h、 12h 、24h、48h after processing.Hematoxylin-eosin （ HE） staining was used to view morphological changes in the hippocampus, immunohistochemical staining to test the expression of p-tau, and terminal dexynucleotidyl transferase（ TdT）-mediated dUTP nick end labeling（ TUNEL） to test neurone apoptosis.Results：In the sham operation group,there were some p-tau expressions and seldom apoptosis neurone in the periventricular white matter; In HIBD group ,the expression of the positive cells of p-tau at each time point were more than that in the sham operation group,differences are statistically significant（F=95.67,p=0.000〈0.05）;the neurone apoptosis numbers were more than that in the sham operation group too, differences are statistically significant（F=18.829,p=0.001〈0.05）.Conclusion：In HIBD group, the expression of p-tau pro-tein and the numbers of neurone apoptosis were obvious than the sham one.

关 键 词：缺氧缺血性脑损伤 TAU蛋白 凋亡 微管相关蛋白 

分 类 号：R493[医药卫生—康复医学]