二甲基亚砜充当保护剂灌注兔离段肢体局部药物浓度的相对回收率  

Dimethyl sulfoxide acts as a protective agent to perfuse rabbit amputated limbs: the relative recovery of local drug concentrations

作　　者：安卓玲[1] 丁琨[2] 刘春生[2] 朱泽兴[2] 周静超 张朕[2] 张树明[2] 王丹[2] 

机构地区：[1]首都医科大学附属北京朝阳医院,北京市100020 [2]解放军第二炮兵总医院,北京市100088

出　　处：《中国组织工程研究》2015年第24期3855-3859,共5页Chinese Journal of Tissue Engineering Research

基　　金：全军十一五医药卫生科研基金项目(08G051);国家自然科学基金项目(81301662;81341015)~~

摘　　要：背景:二甲基亚砜冷冻保护剂的效果在低温医学应用中得到了大量验证,目前尚无利用微透析技术对二甲基亚砜进行检测的文献报道。目的:考察不同浓度断肢再植冷冻保护剂二甲基亚砜微透析的相对回收率。方法:应用增量法及减量法检测体外不同浓度(2%、5%、8%)二甲基亚砜微透析线性探针的相对回收率,并进行体内校正实验,以减量法估算二甲基亚砜在兔离断肢体的相对回收率。结果与结论:增量法检测2%、5%、8%浓度二甲基亚砜的相对回收率分别为(49.49±3.56)%,(46.30±1.48)%,(52.66±2.54)%,平均回收率为(49.48±3.18)%;减量法检测2%、5%、8%浓度二甲基亚砜的相对回收率分别为(50.99±6.89)%,(43.86±1.35)%,(50.67±0.75)%,平均回收率为(48.51±4.03)%,两种方法检测的二甲基亚砜微透析探针相对回收率基本相同。体内校正实验显示回收率随二甲基亚砜浓度的变化较小,平均回收率为(15.45±4.80)%。结果表明不同浓度二甲基亚砜的微透析探针相对回收率无明显差异。BACKGROUND：The effect of dimethyl sulfoxide cryoprotectants has been got a lot of verification in the low-temperature medical applications. But there is no literature addressing microdialysis detection of dimethyl sulfoxide cryoprotectants. OBJECTIVE：To investigate the microdialysis relative recovery of different concentrations of dimethyl sulfoxide cryoprotectants used for limb reattachment. METHODS：In vitro linear probe relative recovery of different concentrations of dimethyl sulfoxide （2%, 5%, 8%） was detected by retrodialysis gain and loss method. The correction in vivo experiment was done to estimate dimethyl sulfoxide relative recovery in rabbit amputated limbs. RESULTS AND CONCLUSION：The relative recoveries of different concentrations of dimethyl sulfoxide （2%, 5%, 8%） were （49.49±3.56）%, （46.30±1.48）%, （52.66±2.54）%using retrodialysis gain method and （50.9±6.89）%, （43.86±1.35）%, （50.67±0.75）%using retrodialysis loss method. The average recoveries were （49.48±3.18）%and （48.51±4.03）%, respectively. There was no difference in the relative recovery of dimethyl sulfoxide detected using two methods. The change of dimethyl sulfoxide concentration could not influence the retrodialysis gain and loss method calibration results. The recovery was （15.45±4.8）%in vivo. These findings indicate that the microdialysis technology is suitable for dimethyl sulfoxide sampling in vivo that has no obvious influence on the relative recovery.

关 键 词：二甲亚砜 组织构建 组织工程 二甲基亚砜 微透析 相对回收率 校正 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]