Effect of Cultural Conditions on Chitinase Production from Biocontrol Bacterium Against Aflatoxin  

作　　者：Kai Wang Peisheng Yan Lixin Cao 

机构地区：[1]School of Municipal and Environmental Engineering,Harbin Institute of Technology [2]School of Marine Science and Technology,Harbin Institute of Technology

出　　处：《Journal of Harbin Institute of Technology(New Series)》2015年第3期82-86,共5页哈尔滨工业大学学报（英文版）

基　　金：Sponsored by the National Natural Science Foundation of China(Grant No.30870003,21273056);the Foundation for Outstanding Yong Scientist in Shandong Province(Grant No.BS2014NY012)

摘　　要：Chitinase is one of the most important mycolytic enzymes with industrial significance. Statistical methods are employed to optimize cultural conditions with the increased production of chitinase for the selected Serratia marcescens JPP1,which are obtained from peanut hulls in Jiangsu Province, China and exhibit antagonistic activity against aflatoxins. Using single-factor experiments the effects of cultural conditions( broth content,inoculum size and rotation speed) on chitinase production from S. marcescens JPP1 are evaluated. Central composite design of Response Surface Methodology is used to optimize the levels of factors for the best yield of enzymes production. The optimized cultural conditions for obtaining the highest level of chitinase production are23. 2 mL broth content,116 r / min rotation speed and 4. 3% inoculum size. A quadratic regression model of chitinase production is built( R2= 0. 970 9) and the verification experiments confirm its validity. The maximum chitinase production obtained after the optimization is 29. 58 U / mL for a 1. 4-fold increase.Chitinase is one of the most important mycolytic enzymes with industrial significance. Statistical methods are employed to optimize cultural conditions with the increased production of chitinase for the selected Serratla marcesces JPP1, which are obtained from peanut hulls in Jiangsu Province, China and exhibit antagonistic activity against aflatoxins. Using single-factor experiments the effects of cultural conditions （broth content, inoeulum size and rotation speed） on chitinase production from S. mareeseens JPP1 are evaluated. Central composite design of Response Surface Methodology is used to optimize the levels of factors for the best yield of enzymes production. The optimized cultural eonditions for obtaining the highest level of ehitinase production are 23.2 mL broth content, 116 r/rain rotation speed and 4. 3% inoeulum size. A quadratic regression model of chitinase production is built （R2 = 0. 970 9） and the verification experiments confirm its validity. The maximum chitinase production obtained after the optimization is 29. 58 U/mL for a 1.4-fold increase.

关 键 词：CHITINASE AFLATOXIN optimization Central Composite Design 

分 类 号：X56[环境科学与工程—环境工程]