小鼠骨髓源性树突状细胞体外诱导培养体系的构建  

A culture system for dendritic cells induced from murine bone marrow in vitro

作　　者：刘振明[1] 胡何节[1] 方征东[1] 王晓天[1] 孙小杰[1] 葛新宝[1] 

机构地区：[1]安徽医科大学附属省立医院普通外科,合肥230001

出　　处：《安徽医科大学学报》2015年第8期1177-1180,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1208085MH151;1408085MH177)

摘　　要：建立一种体外诱导培养小鼠骨髓源性的树突状细胞(DCs)方法,在镜下可观察到培养出的未成熟树突状细胞(im DCs)比成熟树突状细胞(m DCs)细胞突起少。流式细胞术检测CD80、CD86、MHC-Ⅱ分子在im DCs的表达显著低于在m DCs上的表达。同种异基因混合淋巴细胞反应显示DCs刺激T细胞增殖的能力随着DCs所占比例的增大而增强,在相同比例条件下对刺激T细胞增殖的能力,im DCs显著低于成熟m DCs。该诱导方法可获得大量符合科研要求的小鼠骨髓源性DCs。To establish a culture system for dendritic cells（DCs） induced from murine bone marrow in vitro. Com-pared with mature DCs, fewer spines were observed in immature DCs under microscopy. The expressions of cell surface molecules in immature DCs were significantly lower than those of mature DCs. MLR indicated T cells prolif-eration capacities of same-reaction ratio in immature DCs were significantly lower than those in mature DCs. The methods of culturing DCs establised by us can be used in the future experment.

关 键 词：小鼠 树突状细胞 细胞培养 

分 类 号：R654.3[医药卫生—外科学]