利用定量加压充气球囊制备大鼠心肌缺血再灌注损伤模型  

Establishment of rat model of myocardial ischemia-reperfusion injury by using a quantitative inflating saccule

作　　者：许士进 何淑芳[1] 金世云[1] 胡军[1] 张野[1] 

机构地区：[1]安徽医科大学第二附属医院麻醉科,合肥230601

出　　处：《安徽医科大学学报》2015年第8期1180-1183,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81471145)

摘　　要：雄性SD大鼠随机分为3组,每组20只:假手术组(SHAM组)、球囊缺血再灌注组(SI/R组)、经典缺血再灌注组(I/R组)。3组均在冠状动脉左前降支下穿线,SHAM组仅将未充气的球囊固定于穿线处;SI/R组固定球囊后,通过加压泵对球囊充气和放气建立缺血再灌注损伤模型;I/R组采用传统的造模方法。记录血流动力学和心电图的特异性改变,再灌注2 h后取心脏进行TTC染色测心肌梗死面积。结果显示,SI/R和I/R组均可造成显著心肌缺血,两组死亡率和心肌梗死体积(IS/ARR)的差异无统计学意义。Adult male SD rats were randomly divided into three groups, 20 rats in each group： Sham operation group （SHAM group）, saccule ischemia-reperfusion group （ SI/ R group）, classical ischemia-reperfusion group （I/ R group）. A 5-0 silk tread was passed around the left anterior descending coronary artery of rat heart in all of the three groups. In SHAM group, the saccule was fixed on the artery without inflation. In SI/ R group, the fixed saccule was inflated and then deflated to induce myocardium ischemia and reperfusion by using a pressure pump. In I/ R group, the classical method was used to establish the model. Hemodynamic parameters and electrocardiograph-ic changes were recorded during the experiment. Infarct size （IS）, as a percentage of the area at risk （AAR）, was determined by 2,3,5-triphenyltetrazolium （TTC） staining at the end of reperfusion. The results showed regional is-chemia was induced in both SI/ R and I/ R groups, and there was no statistically significant difference between SI/R and I/ R groups regarding animal mortality and infarct size （IS / ARR）.

关 键 词：动物模型 心脏 缺血再灌注 充气 球囊 

分 类 号：R654.2[医药卫生—外科学] R-332[医药卫生—临床医学]