羧基化聚乙烯基萘纳米微球的制备和应用  

Preparation and application of carboxylic polyvinyl naphthalene nanospheres

作　　者：张俊峰[1] 靳颖[2] 徐亮[3] 程帅[3] 王冬梅[3] 王彦春[1] 魏殿军[1] 

机构地区：[1]天津医科大学第二医院检验科,天津300211 [2]武警后勤学院附属医院,天津300163 [3]天津市临床药物关键技术重点实验室天津医科大学药学院,天津300070

出　　处：《安徽医科大学学报》2015年第8期1184-1188,共5页Acta Universitatis Medicinalis Anhui

基　　金：天津市应用基础与前沿技术研究计划(编号:14JCYBJC24300)

摘　　要：以乙烯基萘(VN)为聚合单体,采用乳液聚合法制备聚乙烯基萘(PVN)纳米微球。通过对其羧基化与β2微球蛋白(β2-M)抗体偶联,制成免疫检测试剂。分别用聚苯乙烯(PS)和PVN检测试剂测定β2-M含量,数据经统计学处理和分析,对自制的PVN纳米微球胶乳增强免疫比浊(LETIA)检测试剂的性能进行评价。自制的羧基化PVN纳米微球免疫试剂成功对标准样本进行了检测并在一定范围内有较好的线性,比PS检测试剂更加灵敏。利用该研究采用的方法和条件可以成功制备粒径大小可控、单分散的羧基化PVN纳米微球,是比PS更优的LETIA新载体,具有很好的临床应用前景。Using vinyl naphthalene（VN） as the monomer, polyvinyl naphthalene（PVN） nanometer microspheres were synthesized by emulsion polymerization,which were made for immunodetection reagents by carboxylating and coating with β2-microglobulin（β2-M） antibody. β2-M was determined respectively using polystyrene（PS）and PVN immunodetection reagents and the results were statistically processed and analyzed,the purpose of which was that the performance of PVN-homemade nanoparticles carrier for latex enhanced turbidimetric immunoassay （LETIA） was evaluated. Immunological reagents of carboxylated PVN nanospheres had been successfully used for testing standard samples and having good linearity,therefore they were more sensitive than that of PS nanospheres. Control-lable particle size, monodisperse carboxylated PVN nanoparticles can be successfully synthesized by using method and conditions of this paper and have good prospects for clinical application,which are better than that of PS nano-spheres carrier for LETIA.

关 键 词：乙烯基萘 胶乳增强免疫比浊法 Β2微球蛋白 纳米微球 

分 类 号：R318.08[医药卫生—生物医学工程] R313[医药卫生—基础医学]