自体脂肪间充质干细胞移植对糖尿病大鼠肾功能的改善作用  

Autologous transplantation of adipose-derived mesenchymal stem cells improves nenal function in diabetic rats

作　　者：闵小春[1] 伍婷婷[1] 杞少华[2] 姚维琪 武栋成[2] 

机构地区：[1]华中科技大学同济医学院附属普爱医院,武汉市430033 [2]武汉大学基础医学院生物化学与分子生物学系,430071

出　　处：《实用医学杂志》2015年第13期2115-2118,共4页The Journal of Practical Medicine

基　　金：武汉市卫计委科研基金资助项目(编号:WX13D03;WX13C17)

摘　　要：目的:利用动物模型研究自体脂肪间充质干细胞(adipose-derived mesenchymal stem cells,ADMSCs)移植对糖尿病大鼠肾功能的改善作用。方法 :SD大鼠连续5 d腹腔注射40 mg/kg链脲佐菌素(streptozotocin,STZ)建立1型糖尿病动物模型。造模4周后随机将18只SD大鼠分为糖尿病组(n=9)和ADMSCs移植组(n=9),另选正常大鼠作为对照组(n=9)。取自体ADMSCs经体外培养、鉴定后,尾静脉注射到ADMSCs组大鼠体内。于ADMSCs移植8周后测定各组大鼠血糖、胰岛素、血尿素氮、血肌酐和24 h尿蛋白的水平,并测量体重、肾重。结果:ADMSCs经体外培养后表达间充质干细胞表型抗原,并能够诱导分化为成骨细胞和成脂细胞。糖尿病组和ADMSCs组大鼠的血糖、尿素氮、血肌酐、24 h尿蛋白及肾重/体重比值均明显高于对照组(P<0.05)。ADMSCs组大鼠的血糖、尿素氮及肾重/体重比值均低于糖尿病组(P<0.05),且胰岛素水平较糖尿病组有所升高(P<0.05)。另外,ADMSCs组较糖尿病组的24 h尿蛋白和血肌酐水平下降,但差异无统计学意义。结论:自体ADMSCs移植能够在一定程度上改善和缓解1型糖尿病大鼠的肾功能紊乱。Objective To investigate the renoprotective effects of autologous transplantation of adipose- derived mesenehymal stem cells （ADMSCs） in diabetic rats. Methods Sprague-Dawley （SD） rats were injected intraperitoneally with 40 mg/kg streptozotocin （STZ） for 5 consecutive days to induce type 1 diabetes. Four weeks following STZ injection, eighteen SD rats were randomized into two groups： the diabetic group （n = 9） and the ADMSCs group （n = 9）. Normal nondiaetic rats were set as the normal control （n = 9）. Autologous ADMSCs were cultured and identified in vitro, which were intravenously injection to the ADMSCs group rats via the tail vein. At 8 weeks after transplantation, levels of blood glucose, insulin, serum urea nitrogen, serum creatinine and urine protein were measured. Meanwhile the body weight and kidney weight were examined. Results Mesenchymal cell surface markers were expressed in the cultured ADMSCs. The ADMSCs could differentiate into the adipogenic and osteoblastic lineages. Both the diabetic group and the ADMSCs group rats had higher levels of blood glucose, urea nitrogen, serum creatinine, urine protein and higher ratio of the kidney weight/body weight than those in the normal control group （P 〈 0.05, respectively）. Blood glucose, urea nitrogen and the ratio of kidney weight/body weight in the ADMSCs group rats were significantly decreased compared with the diabetic group （P 〈 0.05, respectively）. The decreased insulin level was attenuated after transplantation of ADMSCs （P 〈 0.05）. Besides, levels of serum creatinine and urine protein in the ADMSCs group were lower than those in the diabetic group with no significant difference. Conclusion Autologous transplantation of ADMSCs can improve metabolic disorder and relieves diabetic renal damage.

关 键 词：糖尿病肾病 脂肪间充质干细胞 肾功能 

分 类 号：R587.2[医药卫生—内分泌]