脂肪源干细胞-聚丙烯复合网片的生物相容性实验研究  

Experimental study on the in vivo biocompatibility of polypropylene mesh scaffolds with adipose-derived stem cells in rabbits

作　　者：程慧[1] 张蓓[1] 成杰[1] 曹义娟[1] 

机构地区：[1]徐州市中心医院妇产科,徐州医学硕士221009

出　　处：《医学研究生学报》2015年第7期692-695,共4页Journal of Medical Postgraduates

基　　金：江苏省医学重点人才基金(RC2011047);徐州市中心医院创新团队科技项目(xzs2012036)

摘　　要：目的如何避免合成补片与人体组织产生的排异反应是目前急需解决的问题,文中探讨脂肪源干细胞(adipose derived stem cells,ADSCs)与聚丙烯网片在动物体内的生物相容性。方法制备兔脂肪源性干细胞悬液。ADSCs传代扩增后,接种到聚丙烯网片支架上,体外培养1周。分别将聚丙烯网片、ADSCs-聚丙烯复合网片植入家兔的腹直肌表面,4周后观察网片腐蚀、粘连情况,HE染色进行组织学观察,采用RT-PCR检测VEGF mRNA的表达水平。结果流式细胞仪检测结果提示ADSCs CD90、CD44、CD73、CD45、CD34及CD14表达分别为98.54%、95.32%、98.49%、1.21%、3.01%、2.14%。聚丙烯网片、ADSCs-聚丙烯复合网片均有不同程度的腐蚀、粘连,但聚丙烯网片粘连更致密。与聚丙烯比较,ADSCs-聚丙烯复合网片诱导慢性炎症反应轻,炎症评分降低[(1.1±0.2)分vs(0.6±0.1)分,P=0.001],新生血管形成评分升高[(1.7±0.0)分vs(2.6±0.3)分,P=0.000],成纤维细胞增殖评分升高[(0.9±0.1)分vs(2.2±0.2)分,P=0.001]。ADSCs-聚丙烯复合网片周围组织VEGF mRNA含量高于聚丙烯网片(t=94.6,P<0.05)。结论 ADSCs-聚丙烯复合网片具有良好的生物相容性,可作为脂肪组织工程较理想的生物支架材料。Objective How to avoid the rejection of the synthetic patch and human tissue has become an urgent problem to be solved.The article investigated thein vivobiocompatibility of polypropylene mesh scaffold with adipose -derived stem cells（ADSCs） in rabbits. Methods Rabbit ADSC suspension were prepared.ADSCs were seeded onto polypropylene mesh scaffolds after passage and amplification and cultured invitro for 1 week .The polypropylene mesh and ADSC fixed polypropylene mesh were implanted respec-tively into the surface of rectus abdominis in rabbits.4 weeks later, adhesion and erosion of the meshes were evaluated, HE staining was used in histological observation and RT-PCR was applied to detect the dynamic changes of VEGF mRNA level.ADSCs were isola-ted from rabbit subcutaneous adipose tissue after collagenase digesting, filtrating and centrifuging. Results The results of flow cy-tometry showed that the expressions of CD44, CD73, CD90, CD45, CD14 and CD34 were 98.54%, 95.32%, 98.49%, 1.21%, 3.01%, 2.14%, respectively.Polypropylene mesh, ADSC-fixed polypropylene mesh had different degrees of corrosion and adhesion , but polypropylene mesh showed denser adhesion.In comparison with polypropylene,ADSC fixed polypropylene meshes induced a mil -der chronic inflammation response,with lower scores for inflammation （1.1 ±0.2 vs 0.6 ±0.1, P=0.001）, higher scores for neovas-cularization （17.0 ±0.0 vs 2.6 ±0.3, P=0.000） and fibroblastic proliferation（0.9 ±0.1 vs 2.2 ±0.2, P=0.001）.Relative a-mounts of VEGF mRNA of were significantly lower for ADSC-fixed polypropylene compared with the remaining polypropylene meshes （t=94.6, P〈0.05）. Conclusi on Polypropylene mesh scaffold with ADSCs exhibits excellent cellular compatibility and have a bright future in clinical practice.

关 键 词：脂肪源干细胞 组织工程 聚丙烯网片 生物相容性 

分 类 号：R711[医药卫生—妇产科学]