不同产地和采收期塔里木马鹿茸多肽的含量测定  

Determination of Antler Peptides in Cervus elaphus yarkandensis from Different Locations and Harvest time

作　　者：吕秀华[1] 周凡 马芹 

机构地区：[1]新疆医科大学,乌鲁木齐830054 [2]新疆华世丹药物研究有限责任公司,乌鲁木齐830011

出　　处：《中国药学杂志》2015年第14期1233-1236,共4页Chinese Pharmaceutical Journal

基　　金：十二五国家科技支撑计划资助项目(2011BAI05B03)

摘　　要：目的 测定不同产地、采收期塔里木马鹿鹿茸多肽的含量,为塔里木马鹿鹿茸的质量评价提供依据。方法 采用牛血清标准蛋白定量试剂盒测定鹿茸多肽中的蛋白含量,比较鹿茸多肽的蛋白含量差异与产地差异和采收期差异。结果 鹿茸多肽在20-2000μg·mL^-1内线性关系良好（r=0.9979）,平均加样回收率为99.06%,RSD为2.08%（n=6）,不同产地鹿茸药材中鹿茸多肽含量存在差异,同一产地不同采收期的鹿茸多肽蛋白含量也存在差异。结论 测定方法简便、准确、重复性好,可作为鹿茸多肽的含量测定方法。OBJECTIVE To determine the contents of antler peptides in Cervus elaphus yarkandensis from different locations and harvest periods to evaluate their qualities. METHODS The concentration of antler peptides of Cervus elaphus yarkandensis was measured by BCA protein assay kit. The protein contents between differen regions and harvest time were compared. RESULTS A good linear relationship was achieved for the calibration curve of antler peptides in the range of 20 -2 000 μg·mL^-1 （r =0. 997 9）. The average recovery was 99. 06% with RSD of 2. 08% （n = 6 ）. The contents of antler peptides in Cervus elaphus Linnaeus from different locations were significantly different. There were also differences in the antler peptides content of the same origin samples collected in different periods. CONCLUSION The established method is simple, accurate and reproducible, which can be adopted for determination of antler peptides in Cervus elaphus Linnaeus .

关 键 词：塔里木马鹿 鹿茸多肽 含量测定 

分 类 号：R284[医药卫生—中药学]