多指标定量指纹图谱在中药金银花质量评价中的应用  

Quality Control of Lonicerae Japonicae Flos by Multi-Marker Components Quantitative Fingerprint

作　　者：何兵[1] 刘艳[1] 李春红[1] 田吉[1] 杨世艳[2] 

机构地区：[1]泸州医学院药物研究中心,四川泸州646000 [2]泸州医学院附属中医医院,四川泸州646000

出　　处：《中国药学杂志》2015年第14期1237-1242,共6页Chinese Pharmaceutical Journal

基　　金：泸州市科技局-泸州医学院2014联合基金资助项目(2013LZLY-K61);泸州市2010年重点科技项目资助项目(泸市财企[2010]41号);泸州医学院2010年青年基金资助项目(泸医院[2010]108号)

摘　　要：目的 探讨多指标定量指纹图谱在中药金银花质量评价中的应用。方法 以绿原酸为参照物建立金银花HPLC指纹图谱,同时以斜率校正法建立绿原酸与其余8个成分（新绿原酸、隐绿原酸、当药苷、芦丁、木犀草苷、异绿原酸B、异绿原酸A、异绿原酸C）的相对校正因子,计算其含量,实现一测多评。同时采用外标法测定该9个成分的含量,并比较二者的差异,以验证一测多评法的准确性和可行性。结果 建立了金银花HPLC特征指纹图谱共有模式,标定了21个共有峰,指认了其中9个共有峰,20批金银花的相似度在0.959-0.997间。20批金银花中9个成分的计算值与实测值间无显著差异。结论 采用多指标定量指纹图谱的质量控制模式评价金银花的质量是可行的,准确的,有望成为适合中药特点的质量评价模式。OBJECTIVE To establish a method for the quality control of Lonicerae Japonicae Flos by multi-components quantitative fingerprint. METHODS Chlorogenic acid was selected as the marker of ingredients to establish the HPLC fingerprint and as the internal reference standard to determine the contents of other eight components（ neoehlorogenie acid, cryptochlorogenic acid, chiratin, rutin, galuteolin, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C） according to the relative correction factor of quantitative analysis of multi-components by single marker（QAMS） by slope correction method. At the same time, the nine components were determined by external standard method. The accuracy and feasibility of QAMS were evaluated by comparison of the results between external standard method and QAMS. RESULTS The fingerprint of Lonicerae Japonicae Flos was established, 21 common peaks were identified in all the tested samples, nine of which were verified, the similarities of 20 batches of Lonicerae Japonicae Flos samples were in the range of 0. 959-0. 997, and there was no significant difference between the quantitative test results of nine ingredi- ents in 20 batches by the QAMS method and external standard method. CONCLUSION The method of multi-marker components quantitative fingerprint is feasible and accurate for the quality control of Lonieerae Japonicae Flos, and it might be a new quality evalua- tion pattern for Traditional Chinese medicine.

关 键 词：多指标定量 指纹图谱 金银花 绿原酸 当药苷 芦丁 木犀草苷 

分 类 号：R917[医药卫生—药物分析学]