远志提取物对阿尔采末病模型大鼠学习记忆能力的影响  

Effects of extracts from radix polygalae on learning and memory ability of Alzheimer's disease rats

作　　者：樊云峰[1] 

机构地区：[1]天津市武清区中医院,天津301700

出　　处：《中国新药与临床杂志》2015年第7期517-521,共5页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的观察远志提取物对Aβ25-35致阿尔采末病(AD)大鼠学习记忆障碍的影响,并探讨其可能的机制。方法制备远志水煎液提取物;大鼠海马区注射Aβ25-35建立痴呆大鼠学习记忆障碍模型,设置正常对照组,模型组,远志提取物低、中、高剂量(0.5、1、2 g·kg-1·d-1)组,连续灌胃给药12周,采用Morris水迷宫进行学习记忆能力的行为学检测,Western blot法检测AD大鼠脑组织海马区p53、cleaved-caspase-3的蛋白表达,测定大鼠脑组织海马区超氧化物歧化酶(SOD)、单胺氧化酶-B(MAO-B)的活力以及丙二醛(MDA)的含量。结果 Morris水迷宫结果实验显示,远志提取物各剂量组AD大鼠的定位航行逃避潜伏期和游泳路程较模型组显著降低(P<0.05),游过平台所在象限的次数和在平台所在象限的时间占总时间的比例t/t总显著升高(P<0.05)。与模型组相比,远志提取物各剂量组AD大鼠模型脑组织海马区p53、cleaved-caspase-3蛋白的表达显著降低(P<0.05),SOD的活性增加(P<0.05),MAO-B活性和MDA的含量降低(P<0.05)。结论远志提取物能显著提高Aβ25-35致AD大鼠的学习记忆能力,其机制可能是抑制凋亡、抗氧化作用有关。AIM To explore the effects of extracts from radix polygalae on the learning and memory ability of Alzheimer＇ s disease （AD） rats and its possible mechanism. METHODS Decoction extracts from radix polygala were prepared. AD models were made by inject Aβ25-35 at the hippocampus of rats. The AD rats were divided into the model group, extracts from radix polygala low, middle and high dose group （0.5, 1, 2 g· kg-1·d-1）, set the blank group at the same time. The groups were continuous intragastric administration for 12 weeks. The Morris assay was used to observe the ability of learning and memory of AD rats. The activity of SOD, MAO-B and MDA were detected according to the checkerboard. The effects of extracts from radix polygala on the expression of p53 and cleaved-caspase-3 were explored using western blot assay. RESULTS Compared with the model group, the escape latency and average swimming distance of AD rats were reduced significantly and thefrequency of passing over the quadrant of the platform fixed and t/ttotal were elevated in extracts from radix polygala groups （P 〈 0.05）. The protein expression of p53 and cleaved-caspase-3 were reduced in the extracts from radix polygala groups （P 〈 0.05） , and the activities of MAO-B and SOD, the contents of MDA were reduced （P 〈 0.05）. CONCLUSION The extracts from radix polygala can improve the ability of learning and memory of AD rats, which may be related to the decreased expression of p53, cleaved-caspase-3, MAO-B, MDA and reduce the activity of SOD.

关 键 词：远志 植物提取物 阿尔茨海默病 大鼠 记忆障碍 细胞凋亡 

分 类 号：R965[医药卫生—药理学] R971[医药卫生—药学]