一种胶体金免疫层析试条检测犬利什曼原虫感染的效果评价  

Evaluation of colloid gold labeled immunochromatographic strip test for rapid detection of canine leishmanial infection in China

作　　者：冯晓平[1] 石锋[1] 杨玥涛[1] 高春花[1] 汪俊云[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,国家级热带病国际联合研究中心,世界卫生组织疟疾,血吸虫病和丝虫病合作中心

出　　处：《国际医学寄生虫病杂志》2015年第4期189-192,共4页International JOurnal of Medical Parasitic Diseases

基　　金：国家科技重大专项(2012ZX10004-220,2012ZX10004-201)~~

摘　　要：目的评价检测内脏利什曼病特异抗体的胶体金免疫层析试条检测我国动物源型内脏利什曼病流行区犬血样的效果。方法以杜氏利什曼原虫前鞭毛体粗抗原作为包被抗原．以链球菌G蛋白标记胶体金制成检测特异抗体的免疫层析试条（immunochromatographicstriptest．ICT）。用该试条检测甘肃省陇南市武都区（动物源型黑热病流行区）和甘肃省漳县（非流行区）犬的腿弯处静脉血样品。同时用镜检法、PCR和酶联免疫吸附试验（enzyme．1inkedimmunosorbentassay，ELISA）分别检测犬骨髓、静脉血和血清样本，并比较各种检测方法的敏感性差异。结果对动物源型内脏利什曼病流行区的105头犬的骨髓涂片镜检，阳性检出率为7．62％（8／105）。应用PCR法的阳性检出率为48．57％（51／105）；检测非流行区30份犬静脉血，1份为阳性，阳性检出率为3-33％（1／30）。试条法和ELISA法检测内脏利什曼病流行区的105份犬血样的阳性检出率分别为32-38％（34／105）和33．33％（35／105）：检测内脏利什曼病非流行区的30份犬血样均为阴性。镜检法的阳性检出率低于PCR法、试条法和EUSA法（x^2=43．58、20．12、21．32，P〈0．05），PCR法的阳性检出率高于镜检法、试条法和EUSA法（x^2=43．58、5．71、5．04，P〈0．05），试条法和EUSA法阳性检出率无统计学差异（x^2=0．02。P〉0．05）。结论快速检测内脏利什曼病的胶体金免疫层析试条适用于检测动物源型内脏利什曼病流行区犬血样中特异性抗体。Objective To evaluate a colloid gold immunochromatographic strip test（ICT） for detection of canine visceral leishmaniasis. Methods The streptococcal protein G （SPG） was conjugated with colloid gold as detecting reagent, Leishmania donovani antigen and anti-SPG IgY were immobilized on nitrocellulose in proper position, respectively. The dog samples from an endemic area in Longnan city, Wudu district of Gansu province and a non-endemic area of zoonotic visceral leishmaniasis in Zhang Xian County of Gansu province were collected and subjected to detection by the immunochromatographic strip test （ICT）, mi- croscopy, PCR, and enzyme-linked immunosorbent assay（ELISA） respectively. The sensitivity of above detec- tion methods was compared. Results It was found that the positive detection rates of dog bone marrow sam- pies from endemic area by microscopy and those of venous blood samples by PCR were 7.62%（8/105） and 48.57% （51/105） respectively while those of blood samples by ICT and ELISA were 32.38% （34/105） and 33.33%（35/105） respectively. The results indicated that the microscopic examination detected less positive of the samples than the PCR test, the ICT and the ELISA （x^2=43.58, 20.12, 21.32,P〈0.05）. The results also in-dicated that the PCR test detected more positive of the samples than the microscopic examination, the ICT and the ELISA （x^2=43.58, 5.71, 5.04 ,P〈0.05 ）. There was no statistical difference in the sensitivity between im- munochromatographic strip test and ELISA （x^2=0.02, P〉0.05）. conclusion The colloid gold immunochro- matographic strip test is proved to be a reliable method for detection of leishmanial specific antibody in dogs.

关 键 词：内脏利什曼病 免疫层析试条 犬 检测 评价 

分 类 号：R392.33[医药卫生—免疫学]