柳茶提取物对异烟肼引起的肝损伤小鼠CYP2E1酶活性及mRNA表达的影响  

Effect of Extracts of Sibraea Angustata(Rchd.) Hand-Mazz. on the Enzyme Activity and m RNA Expression of CYP2E1 in Mice with Isoniazid-induced Liver Injury

作　　者：武燕[1] 邓婉蓉[1] 何华[1] 刘健[2] 

机构地区：[1]甘肃中医学院,兰州730030 [2]兰州大学第一附属医院,兰州730030

出　　处：《中国现代应用药学》2015年第7期795-799,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：甘肃中医学院中青年科研基金项目(ZQ2014-6)

摘　　要：目的研究柳茶提取物对异烟肼引起小鼠肝损伤的CYP2E1基因表达的影响。方法 60只小鼠随机分为正常对照组、模型对照组、阳性对照组(水飞蓟宾胶囊)和柳茶提取物低、中、高剂量组(1.0,1.5,2.0 g·kg?1),分别灌胃给予相应的药物,连续10 d,检测各组血清(ALT、AST)及肝脏相关生化指标以及肝脏组织(MDA、SOD、GSH-PX)、病理学变化、CYP2E1酶活性及m RNA表达。结果与模型组比较,柳茶提取物组血清及肝脏相关生化指标有显著改善(P<0.05或0.001),病理学研究进一步证明了其保护作用,CYP2E1的酶活性及m RNA表达均明显降低(P<0.05)。结论柳茶提取物可能通过抑制CYP2E1的活性和表达来减少自由基的产生,从而达到减轻异烟肼引起的小鼠氧化性肝损伤作用。OBJECTIVE To explore the effect of the extracts of Sibraea angustata（ESA） on gene expression of CYP2E1 in mice with liver injury induced by isoniazid（INH）. METHODS Sixty mice were randomly divided into normal control group, model control group, positive control group（Silybin capsules） and three doses（1.0, 1.5, 2.0 g·kg-1） groups of ESA. After intragastric administrated with corresponding drugs for 10 days, the biochemical indicators in surum（AST, ALT） and liver（MDA, SOD, GSH-PX）, histological patterns, the activity and m RNA epression of CYP2E1 in mice were measured and compared. RESULTS Compared with model control group, the activity of related biochemical indexs ameliorated significantiy（P0.05 or 0.001）, which were confirmed by histopathological analysis. The enzyme activity and m RNA expression of CYP2E1 decreased significantly in ESA-treated groups（P0.05）. CONCLUSION ESA may exerts its hepatoprotective activity against INH-induced liver injury by inhibiting the activity and expression of CYP2E1 andproducyion free radical.

关 键 词：柳茶提取物 肝损伤 异烟肼 CYP2E1 

分 类 号：R285.5[医药卫生—中药学]