血清雌二醇标准物质的研制  

Development of Serum 17β-estradiol Reference Material

作　　者：彭絮[1,2] 徐蓓[2] 江红[1] 宋德伟[2] 

机构地区：[1]北京交通大学理学院,北京100044 [2]中国计量科学研究院化学所,北京100013

出　　处：《化学分析计量》2015年第4期1-4,共4页Chemical Analysis And Meterage

基　　金：国家科技基础条件平台工作项目(2011FY130100)

摘　　要：介绍高浓度血清雌二醇标准物质的研制。采集的全血不加任何添加剂和抗凝剂,离心分离出血清,分装。采用同位素稀释–液相色谱串联质谱法(ID–LC–MS/MS)对高浓度血清雌二醇标准物质进行定值及均匀性和稳定性检验,对定值不确定度进行了评定。结果表明,高浓度血清雌二醇标准物质定值结果为3.06 ng/g,扩展不确定度为0.08 ng/g(k=2),均匀性和稳定性符合国家标准物质技术要求。采用IFCC RELA比对样品和欧洲标准局标准物质血清17β-雌二醇标准物质对定值方法进行确认,测量结果的标准偏差在该标准物质量值的不确定度范围内。研制的标准物质量值经过日本计量院验证,测量结果为(3.03±0.1)ng/g。该标准物质的量值具有溯源性和准确性。The development of high concentration of serum 17fl-estradiol reference material was introduced. The serum standard samples were isolated from collected blood by certain conditions before packing, the checking of uniformity, stability test and certified value were systematically studied by ID-LC-MS / MS. The uncertainty of determination was evaluated. The results showed that the certified value of the reference material of high concentration of serum 17 β-estradiol reference material was 3.06 ng / g with an relative expanded uncertainty of 0.08 ng / g （k=2）. The uniformity and stability of the reference material of serum 17β-estradiol could meet the technical requirements of the standard reference material. IFCC RELA sample and European Commission CRM were used for confirmation of dermination method, and the standard deviation of analysis result was in the range of uncertainty. The 17β-estradiol reference material value was proven by the Metrology Institute of Japan, and the detection result was （3.03 ± 0.1） ng/g. The quantity value of the reference material is traceable and accuracy.

关 键 词：血清 雌二醇 同位素稀释-液相色谱串联质谱法 定值 均匀性 稳定性 

分 类 号：O656[理学—分析化学]